吐鲁番黑羊MC1R基因序列分析研究  

Analysis on MC1R Sequences of Turpan Black Sheep

作　　者：於建国[1] 陈童[1] 刘明军[2] 

机构地区：[1]新疆畜牧科学院畜牧研究所,乌鲁木齐830000 [2]新疆畜牧科学院绵羊研究中心,乌鲁木齐830000

出　　处：《中国草食动物科学》2014年第4期16-18,共3页China Herbivore Science

基　　金：新疆自治区科研院所基本科研业务费

摘　　要：为研究黑皮质素受体1(MC1R)基因对吐鲁番黑山羊毛色的影响,试验采用PCR扩增和测序技术,对吐鲁番黑羊MC1R基因编码区核苷酸序列及其与毛色的相关性进行了研究。结果表明:MC1R基因编码区核苷酸序列954 bp,编码317个氨基酸,编码区存在2个错义突变(c.218 T>A,p.73 Met>Lys;c.361 G>A,p.121 Asp>Asn)和3个同义突变(c.429 C>T,p.143 Tyr>Tyr;c.600 T>G,p.200 Leu>Leu;c.735 C>T,p.245 Ile>Ile)。SNPs分析表明,单倍型AATGT数量占62%。初步认为MC1R基因可以作为吐鲁番黑羊黑色被毛调控的候选基因。To study the effects of melanocortin receptor 1（MC1R）on the wool colour in Turpan black sheep,the coding region nucleotide sequence of MC1 R gene of Turpan black sheep and its correlation with the wool colour were studied by using PCR amplification and sequencing techniques. The results showed that the coding region nucleotide sequence of MC1 R gene was 954 bp, encoding317 amino acids, there were two missense mutations（c.218 T〉A,p.73 Met〉Lys;c.361 G〉A,p.121 Asp〉Asn）and three silent mutations（c.429 C〉T,p.143 Tyr〉Tyr;c.600 T〉G,p.200 Leu〉Leu;c.735 C〉T,p.245 Ile〉Ile）in the coding region. SNPs haplotype of AATGT accounted for 62%.It is suggested that the MC1 R gene can be used as the candidate gene in regulating the black wool of Turpan black sheep.

关 键 词：吐鲁番黑羊 MC1R SNPS 候选基因 

分 类 号：S826.2[农业科学—畜牧学]