染料木黄酮对3T3-L1细胞增殖与分化的影响  

Effects of Genistein on Proliferation and Differentiation in 3T3-L1 Preadipocyte

作　　者：何阳[1] 陈东[1] 汪先友[1] 曹兵海[1] 

机构地区：[1]中国农业大学动物科学技术学院,动物营养国家重点实验室,北京100193

出　　处：《中国草食动物科学》2014年第4期26-30,共5页China Herbivore Science

基　　金：国家肉牛牦牛产业技术体系(CARS-38);南方地区草食家畜育肥与高品质肉生产技术研究(201303144)

摘　　要：试验旨在研究染料木黄酮对3T3-L1细胞增殖与分化的影响以及对分化过程中相关基因表达的影响。以浓度为0、10、50、100、200μmol/L的染料木黄酮处理细胞,测定不同天数3T3-L1细胞相对增殖量、细胞合成脂肪量以及第8天与脂肪合成有关的基因PPARγ、FASN、LPL、ACC、HSL的相对表达量。结果表明:在增殖方面,染料木黄酮能显著抑制3T3-L1前脂肪细胞的增殖(P<0.05)。随着染料木黄酮浓度的增加,细胞增殖的抑制作用越强。当浓度为200μmol/L时,细胞停止增殖。在分化方面,染料木黄酮能显著减少3T3-L1前脂肪细胞分化合成脂肪的量(P<0.05)。随着染料木黄酮浓度的增加,细胞分化合成脂肪量减少。染料木黄酮能显著减少3T3-L1细胞与脂肪合成有关的基因PPARγ、FASN、LPL、ACC、HSL的相对表达量(P<0.05)。在浓度为50μmol/L时,PPARγ基因相对表达量最低;在浓度为200μmol/L时,FASN基因相对表达量最低;在浓度为10μmol/L时,LPL基因相对表达量最低;在浓度为200μmol/L时,ACC基因相对表达量最低;在浓度为10μmol/L时,HSL基因相对表达量最低。分析推测,染料木黄酮通过抑制与脂肪合成相关基因的表达来减少脂肪合成量。This experiment was designed to study the effects of genistein on 3T3-L1 cell proliferation and differentiation and its impact on the process of differentiation related gene expression.The cells were treated at the concentration of 0,10,50,100,200μmol/L genistein,and the relative proliferation amount of 3T3-L1 cells on different days,the relative fat synthesize amount of the cells and the related genes relative expression of PPARγ,FASN LPL,ACC,HSL on the 8th day were detected.The results showed that：in terms of proliferation,genistein significantly inhibited the proliferation of 3T3-L1 preadipocytes（P〈0.05）.The higher the concentration of genistein,the stronger the inhibition of cell proliferation.At the concentration of 200 μmol/L,the cells stopped proliferating.In terms of differentiation,genistein significantly reduced the amount of 3T3-L1 preadipocytes fat synthesis（P〈0.05）.With increasing concentrations of genistein,the amount of fat cell differentiation synthesis decreased.Genistein reduced significantly the expression of 3T3-L1 cell＇s relative genes（PPARγ,FASN,LPL,ACC,HSL）（P〈0.05）.At the concentration of 50μmol/L,the relative expression of the PPARγ gene was to the minimum.At the concentration of 200 μmol/L,the relative expression of the FASN gene was to the minimum.At the concentration of 10 μmol/L,the relative expression of the LPL gene was to the minimum.At the concentration of 200 μmol/L,the relative expression of the ACC gene was to the minimum.At the concentration of 10 μmol/L,the relative expression of the HSL gene was to the minimum.Analysis speculated that genistein reduced the amount of fat synthesis by inhibiting the synthesis of fat-relat-ed gene expression.

关 键 词：染料木黄酮 3T3-L1前脂肪细胞 分化 增殖 

分 类 号：S813.1[农业科学—畜牧学]