蕹菜镉积累典型品种根际微生物群落特征研究  

Differences of microbial community characteristics in rhizosphere between low-Cd and high-Cd accumulating cultivars of water spinach(Ipomoea aquatica Forsk)

作　　者：龚玉莲[1] 曾小龙[1] 曾碧健[1] 吕保玉 王俊丽[3] 

机构地区：[1]广东第二师范学院生物系应用生态学实验室,广州510303 [2]广西壮族自治区环境监测中心站,南宁530022 [3]贵阳医学院公共卫生学院,贵阳550004

出　　处：《生态科学》2014年第1期25-31,共7页Ecological Science

基　　金：国家自然科学基金(20877104);广东省自然科学基金(S2012010010456);广东省高等院校学科与专业建设专项资金科技创新项目(2013KJCX0138);广东第二师范学院教授博士专项(2012ARF04)

摘　　要：通过根箱试验研究蕹菜(Ipomoea aquatica Forsk)镉低量积累(QLQ)和高量积累(T308)典型品种根际微生物群落特征。结果表明两个品种根际微生物数量、微生物生物量碳均显著高于非根际(p<0.05)。污染土壤和无污染土壤上T308根际可培养微生物总数、细菌和放线菌数量均显著高于QLQ(p<0.05);而污染土壤上T308根际真菌数量显著低于QLQ(p<0.05)。无污染土壤上QLQ根际微生物生物量碳显著高于T308(p<0.05)。两种土壤上平均颜色变化率(AWCD)均为QLQ高于T308。污染土壤上QLQ根际微生物对BIOLOG Eco板各类碳源的利用程度均高于T308,两个品种根际微生物对不同碳源的利用模式亦不同。表明两个蕹菜典型品种根际微生物群落结构和功能存在显著差异,代谢活性较高可能是QLQ区别于T308的重要根际微生物群落特征。A rhizobox experiment was conducted to investigate microbial community characteristics in rhizosphere soil between two typical eultivars of water spinach （Ipomoea aquatica Forsk）, a low-Cd cultivar （QLQ） and a high-Cd cultivar （T308）. The results showed that populations of bacteria, actinomycetes and fungi and microbial biomass C in rhizosphere soil of water spinach were both significantly higher than those in non-rhizosphere soil （,o〈0.05）. In contaminated and uncontaminated soil, populations of microbe, bacteria and actinomycetes in rhizosphere soil of T308 were significantly higher than those of QLQ （p〈O.05）. However, in contaminated soil, population of fungi in rhizosphere soil of T308 was significantly lower than that of QLQ （p〈0.05）. In uncontaminated soil, microbial biomass C in rhizosphere soil of QLQ was significantly higher than that of T308 （p〈0.05）. The average well color development （AWCD） of QLQ was higher than that of T308. In contaminated soil, utilization efficiency of different carbon source types in BIOLOG Eco plates by rhizosphere microbial community of QLQ was higher than that of T308, and utilization patterns were different between the cultivars. The results indicated that there were differences in microbial community structural and functional characteristics in rhizosphere soil between the typical water spinach cultivars, and the relatively higher metabolic capacity of soil microbial community might be one of the important characteristics that distinguish the low-Cd cultivar QLQ from the high-Cd cultivar T308.

关 键 词：蕹菜 镉积累典型品种 根际 微生物群落特征 

分 类 号：Q938.1[生物学—微生物学]