珠江口萘双加氧酶基因多样性及其与环境因子相关性分析  

Diversity of ndo genes in sediments of the Pearl River estuary in relation to environmental variables

作　　者：吴鹏[1,2] 王友绍[1,2] 张建东[1,2] 

机构地区：[1]中国科学院南海海洋研究所热带海洋环境国家重点实验室,广州510301 [2]中国科学院大亚湾海洋生物综合试验站,深圳518121

出　　处：《生态科学》2014年第2期262-268,共7页Ecological Science

基　　金：中国科学院知识创新工程重要方向性项目(KSCX2-EW-G-12C);国家自然科学基因项目(41176101);"十一五"国家科技支撑计划重点项目(2012BAC07B0402);热带海洋环境国家重点实验室(中国科学院南海海洋研究所)开放课题(LTO1408)

摘　　要：选取珠江口上游到下游的四个站点采用16S rRNA-DGGE方法分析了珠江口表层沉积物中微生物群落结构。结果表明:四个站点中微生物组成差别较大,并且珠江口上游的微生物多样性稍低于下游。基于萘双加氧酶基因(ndo基因)的DGGE分析则表明珠江口上游和下游区域中的ndo基因多样性(站点A1和A4)高于珠江口中游(站点A2和A3)。为了进一步探讨环境因子与ndo基因分布的相关关系,典型对应分析(canonical correspondence analysis,CCA)发现站点A1中ndo基因组成主要受NH4-N影响,该基因在站点A2的分布主要与SiO3-Si正相关,而站点A4的该基因组成则与盐度的相关性较高。研究结果能为珠江口的PAHs生物降解提供重要理论参考。Surface sediments across a four-site transect along the upstream to the downstream of the Pearl River estuary were examined. Bacterial community compositions were firstly characterized using Denaturing Gradient Gel Electrophoresis （DGGE） analysis of bacterial 16S rRNA gene. Bacterial community compositions were quite different in this estuary, and the diversity of bacteria in the upstream was lower than the downstream. Based on the PCR-DGGE analysis of ndo gene, the diversity of ndo gene in the upstream and downstream （sites A1 and A4） was higher than the middle stream （sites A2 and A3）. To further explore the relationships between the distribution of ndo genes and environmental factors, canonical correspondence analysis （CCA） was conducted. The composition of ndo gene at site A1 was significantly influenced by NH4-N, while the concentration of SiO3-Si displayed positive correlation with this gene composition at site A2. However, salinity had a positive influence on the gene composition at site A4. This study can provide for a broader estimation of the PAHs biodegradation potential in this estuary.

关 键 词：多环芳烃 萘双加氧酶 上覆水 典型对应分析 

分 类 号：Q938.1[生物学—微生物学]