亚成体大熊猫肠道真菌的分离与鉴定  

Isolation and Identification of Fungi in Feces of Sub-adult Giant Panda

作　　者：艾生权[1] 夏玉[1] 钟志军[1] 王承东[2] 刘艳红[1] 吴虹林[2] 李才武[2] 杨海迪[2] 林姗姗[2] 石锦江[1] 彭广能[1] 

机构地区：[1]四川农业大学动物医学院,动物疫病与人类健康四川省重点实验室,四川雅安625014 [2]中国林业科学研究院大熊猫保护研究中心,雅安碧峰峡大熊猫基地,四川雅安625007

出　　处：《四川动物》2014年第4期522-527,共6页Sichuan Journal of Zoology

基　　金：国家自然基金面上项目(31272620);教育部"长江学者和创新团队发展规划"创新团队项目(IRT0848);四川农业大学双支计划项目(03570316;03570362)

摘　　要：目的探讨大熊猫肠道真菌菌群结构,为研究肠道真菌对纤维素的降解和控制肠道真菌病奠定基础。方法采用PDA、SDA、高氏3种培养基对8只亚成体大熊猫新鲜粪便进行真菌分离、纯化,经形态学和分子生物学方法进行鉴定。结果经培养获得的真菌主要由4种霉菌(Galactomyces geotrichum占22.74%、Galactomyces reessii占12.37%、Mucor ramosissimus占18.23%和Mucor circinelloides占6.39%)和2种酵母菌(Trichosporon sp.占19.46%和Candida solani strain占20.81%)组成,各样品中真菌所占比例不等。且3种培养基的分离效果显示PDA>SDA>高氏培养基。结论亚成体大熊猫肠道内存在一定比例的可培养真菌。Objective To investigate the fungal community structure in the guts of sub-adult giant pandas, and provide the basis for studying the intestinal fungi to digest cellulose and controlling the fungal disease. Methods In this study, three mediums such as PDA, SDA and Gao’s were chosen to isolate and purify the fungi in fresh feces of sub-adult giant pandas, followed by the combination of morphological and molecular methods for classification. Results The study showed that the fresh faces of eight sub-adult giant pandas were mainly composed of four kinds of mould （Galactomyces geotrichum, 22.74%; Galactomyces reessii, 12.37%; Mucor ramosissimus, 18.23%; Mucor circinelloides, 6.39%） and two kinds of yeast （Trichosporon sp., 19.46%; Candida solani, 20.81%）. The proportions of fungus were different among each sample. The isolation effect of three mediums was： PDA〉SDA〉Gao. Conclusions There are a proportion of culturable fungi in the guts of sub-adult giant pandas.

关 键 词：大熊猫 肠道真菌 分离 鉴定 

分 类 号：S858.9[农业科学—临床兽医学]