成熟培养液中胎牛血清添加量对山羊卵母细胞体外培养成熟的影响  

Effect of Supplemented FBS to the Maturation Medium on the External Mature Culturing of Oocyte from Goats

作　　者：朱广香[1,2] 马恒东[2,3] 张华 

机构地区：[1]四川农业大学生命科学学院,四川雅安625014 [2]四川农业大学动物科技学院,四川雅安625014 [3]四川南充职业技术学院,四川南充637000 [4]四川省雅安市名山区农业局,四川名山625100

出　　处：《四川动物》2014年第4期588-592,共5页Sichuan Journal of Zoology

摘　　要：本研究以屠宰奶山羊的新鲜卵巢为材料,回收可培养卵丘细胞-卵母细胞复合体(COC)。将可培养COC置于M199+FBS+100 IU/mL FSH+100 IU/mL LH培养微滴中,在5%CO2、95%空气、饱和湿度的CO2培养箱中39℃下培养。进行二因子三水平设计,比较基础培养液中添加FBS体积分数为0%、10%和15%时,及培养时间16 h、24 h、26 h的成熟率。结果表明:不添加FBS组的成熟率极显著(P<0.01)低于其他两组,而10%FBS组与15%FBS组差异不显著(P>0.05);体外培养16 h与培养24 h、26 h的卵母细胞成熟率分别为67.6%与81.7%、81.7%,差异极显著(P<0.01),而后二者间未表现差异。This experiment regarded fresh ovary from slaughtered goats as the material. This kind of COC was placed in M199＋the FBS（0%, 10%, 15%） educates liquid＋FSH（100 IU/mL）＋LH（100 IU/mL） educates tiny inside, in the CO2 incubator （5% CO2, 95% air, 100% humidity） under 39℃ temperature descends to educate 16 h, 24 h and 26 h. It is concluded that 10%-15% FBS is beneficial to the maturation in vitro of goat follicular oocytes. The maturation rate of oocytes cultured for 24 h, 26 h was significantly higher than that of 16 h （P〈0.05）.

关 键 词：胎牛血清FBS 卵巢卵母细胞 体外培养成熟 山羊 

分 类 号：S827[农业科学—畜牧学]