猕猴桃溃疡病病原菌分子鉴定与抗性材料初选  

Rapid Molecular Identification of Actinidia Bacterial Canker and Preliminary Screening of Resistant Materials in Kiwifruit

作　　者：张慧琴[1,2] 毛雪琴[3] 肖金平[2] 章镇[1] 谢鸣[2] 

机构地区：[1]南京农业大学园艺学院,江苏南京210095 [2]浙江省农业科学院园艺研究所,浙江杭州310021 [3]浙江省农业科学院植物保护与微生物研究所,浙江杭州310021

出　　处：《核农学报》2014年第7期1181-1187,共7页Journal of Nuclear Agricultural Sciences

基　　金：浙江省果品农业新品种选育重大科技专项(2012C12904-9);浙江省果品产业创新团队项目(2009R50033);浙江省农业厅"三农五方"项目(2010R05A60C01)

摘　　要：为明确我国南方猕猴桃细菌性溃疡病频发区其致病菌的种类与特征,初选出抗性种质材料,以猕猴桃主栽品种红阳、徐香和金丰的溃疡病感病枝条为材料,采用KB培养基、平板划线和梯度稀释法分离病原菌,利用基于细菌16S-23S rDNA内转录间隔区序列设计的特异性鉴别引物,对分离得到的6个代表菌株进行PCR扩增和测序,获得了大小约为280bp的特异性片段,其测序结果与登录号为D86357和AY342165的Pseudomonas syringae pv.Actinidia的序列完全一致。采用离体叶片注射接种、离体枝条针刺接种和活体枝杆针刺接种3种方法鉴定了18份种质材料的抗性。分析结果表明,红阳等中华猕猴桃最感,布鲁诺实生优株13-3、13-4等最抗,美味猕猴桃海沃德居中。本试验所分离的菌株均为丁香假单胞杆菌猕猴桃致病变种(Pseudomonas syringae pv.Actinidia),筛选出2份高抗溃疡病的种质材料。为进一步开展猕猴档细菌性溃疡病防治研究奠定基础。To identify the type and characteristics of bacterial canker of kiwifruit and select the disease-resistant materials,the infected branches of kiwifruit cultivars of A. chinensisHongyang,A. chinensis Jingfeng and A. deliciosa Xuxiang were used,which were collected from Zhejiang,Jiangsu and Jiangxi provinces. For bacteria isolation,plate streaking,gradient dilution and King＇s B media cultivation were used. DNA fragments from six representative strains amplified by polymerase chain reaction（ PCR） primers based on the 16S- 23S rDNA intertranscribed spacer region,were the same as the GenBank accession number of D86357 and AY342165 of Pseudomonas syringae pv. Actinidae. 18germplasm materials were identified for resistance by three methods of in vitro leaves injection inoculation,in vitro shoots needle inoculation and live branches needle inoculation. The results showed that A. chinensis Hongyang found to be the most susceptible,‘13- 4＇and ‘13-3＇selected from the seedlings of A. deliciosa Bruno were the most resistant,and A. deliciosa Hayward was between them. It is concluded that the isolates from the canker infected kiwifruit branches were Pseudomonas syringae pv. Actinidae. Two germplasm materials high resistance to bacterial canker of kiwifruit were selected. This study lays the foundation for further reaserch of prevention and control of kiwifrwit bacterial canker.

关 键 词：猕猴桃 溃疡病 分子鉴定 抗性 筛选 

分 类 号：S436.634[农业科学—农业昆虫与害虫防治]