抗白粉病糯性小麦的多重PCR分子鉴定技术  

Development of PCR-based Molecular Markers for Waxy and Powdery Mildew Resistance in Wheat

作　　者：许立奎[1,2,3] 潘彬荣[1,2,3] 岳高红[1,2,3] 梅喜雪[1,2,3] 刘永安[1,2,3] 张宗宸 周志辉 

机构地区：[1]温州市农业科学研究院,浙江温州325006 [2]浙南作物育种重点实验室,浙江温州325006 [3]温州科技职业学院,浙江温州325006

出　　处：《核农学报》2014年第7期1203-1207,共5页Journal of Nuclear Agricultural Sciences

基　　金：浙江省重大科技专项农业项目(2012C12902-2-3);浙江省旱作粮油科技创新团队项目(2011R50026-13)

摘　　要：为同时鉴定小麦糯性和白粉病抗性,本研究利用已知3对引物建立多重PCR体系,分别扩增WxA1、Wx-B1、Wx-D1和Pm21基因的分子标记,其目的片段大小分别为:230/265、854、204和1400 bp。经过对供试品种和部分F2分离群体的Wx蛋白进行SDS-PAGE电泳检测,其结果与多重PCR结果一致,证明该多重PCR体系准确可靠,可提高小麦糯性兼抗白粉病的选育效率。In order to identify waxy and powdery mildew resistance of wheat at the same time,a multiple-PCR system with three pairs of known primers was established. The molecular markers of Wx-A1,Wx-B1,Wx-D1 and Pm21 genes were amplified,and the size of the target sequences were 230 /265 bp,854 bp,204 bp and 1400bp,respectively. The Wx proteins of involved cultivars and their derived F2segregation population were detected by SDS-PAGE electrophoresis.The results were in agreement with those of multiple-PCR amplification of the three waxy genes,indicating that this system was reliable. The technique of PCR-based molecular identification established in this study can greatly improve selection efficiency of waxy and powdery mildew resistance in wheat.

关 键 词：小麦 多重PCR 白粉病抗性 糯性 

分 类 号：S512.1[农业科学—作物学]