高效阴离子交换色谱分离-积分脉冲安培检测法同时测定烟草薄片中的糖和保润成分  

Determination of sugars and moisturizing ingredients in tobacco sheet using high performance anion exchange chromatography with integrated pulsed amperometric detection

作　　者：张蓉[1,2] 司晓喜 伊奥尔 张红[3] 丁中涛[2] 张凤梅 朱瑞芝 

机构地区：[1]云南中烟工业有限公司技术中心,昆明650231 [2]云南大学化学科学与工程学院,昆明650091 [3]昆明理工大学化工学院,昆明650500

出　　处：《分析试验室》2014年第8期963-966,共4页Chinese Journal of Analysis Laboratory

摘　　要：建立了阴离子交换色谱分离一积分脉冲安培检测分离测定烟草薄片中糖和保润成分的方法，研究了4种糖成分（葡萄糖、半乳糖、果糖和蔗糖）和5种保润成分（丙二醇、丙三醇、木糖醇、山梨醇和甘露醇）在色谱中的保留行为。以480mmol／LNaOH淋洗液等度淋洗，Carbopac MAl阴离子交换色谱柱进行分离，积分脉冲安培检测器检测，在所考察浓度范围内，9种化合物在测试条件下线性关系良好，线性相关系数大于0．996，检出限为12．6～39．9μg／L（以3倍信噪比计算），5次平行进样，相对标准偏差为1．1％～3．3％，标准加标回收率在95．4％～104．8％之间。An anion exchange chromatography with integrated pulsed amperometric detection was established to separate propylene glycol, glycerol, xylitol, sorbitol, mannitol, glucose, galactose, fructose and sucrose in reconstituted tobacco. 480 mmol/L NaOH was used as an eluent, nine compounds in tobacco sheet were separated on a dionex Carbopac MA1 anion exchange column and detected with integrated pulsed amperometric detector. The nine compounds had a good linear relationship and the correlation coefficients of linear regression equations were above 0. 996 in the studied ranges . Under five parallel sampling, the relative standard deviations were in the range of 1.1% to 3.3% and the recoveries ranged from 95.4% to 104.8%. The method is simple, rapid, accurate and reliable, and it is suitable for the detection of sugars and moisturizing ingredients in reconstituted tobacco.

关 键 词：阴离子交换色谱 脉冲安培检测 烟草薄片 糖 保润成分 

分 类 号：O658.1[理学—分析化学]