南极海洋假单胞菌Pseudomonas sp.NJ-013的化学成分  

Secondary metabolites of Pseudomonas sp. NJ-013 from the Antarctic

作　　者：王成[1,2] 杨桥[1] 马丽艳[1] 田晓清[1] 陆亚男[1] 樊成奇[1] 黄洪亮[1] 

机构地区：[1]农业部东海与远洋渔业资源开发利用重点实验室中国水产科学研究院东海水产研究所,上海200090 [2]上海海洋大学,上海201306

出　　处：《海洋渔业》2014年第4期350-356,共7页Marine Fisheries

基　　金：南极周边海域磷虾等生物资源考察与评估(CHINARE2013-01-06);中央级公益性科研院所基本业务费专项(2014M02);上海市自然科学基金(南极磷虾来源海洋药物先导化合物的发现11ZR1450000)

摘　　要：本研究进行了一株南极假单胞菌Pseudomonas sp.NJ-013代谢产物多样性分析、化学成分的分离和结构鉴定,以探讨其产物结构类型。菌株NJ-013甲醇提取物经LC-TOF MS测试分析其代谢产物多样性;还经柱层析和半制备高效液相色谱制备得到4个化学成分;化合物结构经TOF MS、1H-和13C-NMR等波谱学方法和文献数据对照鉴定。假单胞菌Pseudomonas sp.NJ-013的代谢产物主要类型为含氮的生物碱或寡肽类,分离得到的化合物1-4分别鉴定为环(4'-羟基苯丙氨酸-脯氨酸)(1)、环(4-羟基脯氨酸-亮氨酸)(2)、环(4-羟基脯氨酸-苯丙氨酸)(3)、环(异亮氨酸-脯氨酸)(4)。结构较为复杂的微量成分尚待进一步富集、研究。Diversity analysis on secondary metabolites of an Antarctic microbe strain, Pseudomonas sp. NJ- 013, together with the structural elucidation of some purified compounds, was carried out for exploration of its＇ chemical constituents. Pseudomonas sp. NJ-013 was isolated from sea water samples collected from the fishing area of Antarctic krill. After being cultured in GAUZE＇s Medium NO. 1 for 7 d at 28 ℃, Pseudomonas sp. NJ-013 was extracted with acetone/methanol to give a total extract, or absorbed by HP-20 macroporos resin to afford three extract sections NJ-013-A, NJ-O13-B and NJ-O13-C. The methanol solution of the total extract was subjected to HPLC-TOF MS test for diversity analysis on its＇ secondary metabolites. The possible formula of 54 sample peaks were obtained among 68 observed peaks by high-resolution HPLC-TOF MS spectra. The possible formula indicated 41 -43 N-containing compounds, and the other 11 -13 compounds without nitrogen in their structures. There were also 3 metabolites which were both S- and N-containing compounds, but no halogen-containing sample peaks were found by typical MS spectra. Most sample peaks showed very low relative contents with their Vol% much less than 1.0%. The Vol% of only 13 sample peaks were higher than 2.0% , including 7 - 8 N-containing compounds and other 4 peaks without possible formula. So chemical constituents of Pseudomonas sp. NJ-013 were mainly N-containing compounds, including alkaloids and oligopeptides. Four compounds were also obtained from high polarity sections NJ-O13-A and NJ- 013-B of the extract, by different column chromatography and repeated preparative HPLC, and their structures were elucidated by direct comparison of HPLC-TOF MS, tH- and 13C-NMR data with those reported. The structures of four purified compounds were identified as 3-（ 4-hydroxybenzyl ） hexahydropyrrolo [ 1,2-a ] pyrazine-1,4-dione （ 1 ）, 7-hydroxy-3-isobutylhexahydropyrrolo [ 1,2-a ] pyrazine-1,4-dione （2）, 3-benzyl-7- hydroxyl-hexahydropyrrolo [ 1,2-a] pyrazine-1,4

关 键 词：南极微生物 假单胞菌Pseudomonas SP NJ-013 代谢产物 

分 类 号：Q956[生物学—动物学]