辛夷中辛夷脂素与木兰脂素高效液相色谱含量测定方法的建立与优化  

Establishment and Optimization of the Method for Determination of Magnolin and Fargesin in Magnolia biondii Pamp. by HPLC

作　　者：曹纬国[1,2,3] 陶燕铎[4] 颜学伟[1] 张丹[1] 王刚[1] 

机构地区：[1]重庆医科大学中医药学院,重庆400016 [2]重庆医科大学中医药学院中医药研究室,重庆400016 [3]重庆医科大学中医药学院中医药实验教学中心,重庆400016 [4]中国科学院西北高原生物研究所,青海西宁810000

出　　处：《时珍国医国药》2014年第7期1598-1599,共2页Lishizhen Medicine and Materia Medica Research

基　　金：国家"十一五"国家科技支撑计划(No.2007BAI45B00);重庆市卫生局中医药科技项目(No.2010-1-146)

摘　　要：目的建立并优化辛夷中木兰脂素与辛夷脂素的含量测定方法。方法采用HPLC法同时测定辛夷中木兰脂素与辛夷脂素的含量,色谱柱为hypersil ODS2 C18柱(5μm,250×4.6 mm),流动相为甲醇-水(60∶40),流速为1.0 ml·min-1,检测波长为278 nm。结果木兰脂素在30~600μg·ml-1浓度范围内线性关系良好(r=0.999 7),平均加样回收率为98.72%;辛夷脂素在20~400μg·ml-1浓度范围内线性关系良好(r=0.999 5),平均加样回收率为98.64%。结论该方法准确、简便、快速,适用于辛夷中木兰脂素与辛夷脂素的含量分析,可为辛夷药材及其制剂的质量控制提供参考。Objective To establish and optimize the method for determination of magnolin and fargesin in Magnolia biondii Pamp. Methods HPLC method was used. Analyses were carried out on Hypersil ODS2 C^s （250 mm x4.6 mm,5 m） column. The mobile phase was acetonitrile - water at the flow rate of 1 ml. min -1. The wavelength of detector was set at 278nm. Results The standard curve was linear with the range of 30 . 600 g .ml- 1 for magnolin, and the average recovery rate was 98.72%. The stand- ard curve was linear with the range of 20 -400 g . ml- 1 for fargesin, and the average recovery rate was 98.64%. Conclusion The method is simple and rapid, and suitable to determine the content of magnolin and fargesin in Magnolia biondii Pamp. The method can he usad for the qualitv contrnl of Magnolia biondii Pamp.and related preparatin.

关 键 词：辛夷 木兰脂素 辛夷脂素 高效液相色谱法 

分 类 号：R284.2[医药卫生—中药学]