基于抗凝活性评价的健脑益智胶囊中水蛭提取工艺优选  

Optimization of extraction process of hirudo from JianNaoYiZhi Capsule based on antithrombin activity evaluation

作　　者：郭东艳[1] 吕杨[1] 赵晓平[2] 范小璇[2] 侯文[2] 

机构地区：[1]陕西中医学院药学院,陕西咸阳712046 [2]陕西中医学院附属医院,陕西咸阳712000

出　　处：《时珍国医国药》2014年第7期1636-1637,共2页Lishizhen Medicine and Materia Medica Research

基　　金：陕西省科技厅资助项目(No.2011KTCL03-02)

摘　　要：目的优选健脑益智胶囊中水蛭的提取工艺。方法采用正交试验设计法,以抗凝血酶活性作为评价指标,考察乙醇浓度、加醇量、提取时间、提取次数对水蛭提取工艺的影响。结果正交试验优选确定的工艺条件为8倍量60%乙醇提取2次,每次1.5 h。结论健脑益智胶囊中水蛭的提取工艺条件合理、稳定、可行。Objective To optimize the extraction process of hirudo from JianNaoYiZhi capsule. Methods The orthogonal experi- mental design was used to study the influence of extraction of hirudo with ethanol concentration, solvent usage, extracting time, ex- traction times as factors, the activity of anti - thrombin as the evaluation index to optimize the extraction process. Results The op- timum extraction process conditions by the orthogonal experimental design were 60 % ethanol , extracted for 1.5 hours, 8 - fold sol- vent and 2 times of extraction. Conclusion This extraction process conditions of hirudo from JianNaoYiZhi capsule were reasona- ble, stable and feasible.

关 键 词：水蛭 正交试验 提取工艺 

分 类 号：R284.2[医药卫生—中药学]