柱前衍生化高效液相色谱法分析不同生长年限金钗石斛多糖的单糖组成  

Analysis of Monosaccharide Compositions in Polysaccharide of Dendrobium nobile Lind.from Different Growth Years by Precolumn Derivation HPLC

作　　者：许莉[1] 郭力[1] 罗方利[1] 张廷模[1] 刘贤武[1] 韩瑜 

机构地区：[1]成都中医药大学药学院中药材标准化教育部重点实验室/中药资源系统研究与开发利用省部共建国家重点实验室培育基地,四川成都611137 [2]四川万安石斛产业开发有限公司,四川成都610213

出　　处：《时珍国医国药》2014年第7期1725-1727,共3页Lishizhen Medicine and Materia Medica Research

基　　金：科技部"十二五"支撑项目(No.2011BAI13B02-8);四川省科技厅课题(No.2011JY0048)

摘　　要：目的建立柱前衍生化高效液相色谱法分析不同生长年限金钗石斛多糖的单糖组成。方法将金钗石斛多糖样品用三氟乙酸(TFA)水解成单糖后,通过1-苯基-3-甲基-5-吡唑啉酮(PMP)衍生,衍生物采用高效液相色谱梯度洗脱,研究金钗石斛多糖的单糖组成。结果不同生长年限金钗石斛多糖的单糖组成差异较大,一年生多糖由甘露糖、鼠李糖、半乳糖醛酸、葡萄糖、半乳糖、木糖、阿拉伯糖7种单糖组成,其摩尔比为68.33∶1.11∶1.00∶18.96∶3.44∶5.63∶7.26;二年生多糖由甘露糖、半乳糖醛酸、葡萄糖、半乳糖、木糖、阿拉伯糖6种单糖组成,其摩尔比为5.54∶1.07∶26.26∶1.00∶3.15∶5.75。结论该方法具有简便、结果准确等特点,可用于金钗石斛多糖中单糖组成的分析。该研究为金钗石斛的质量评价和开发利用提供了实验依据。Objective To develop a method based on precolumn derivation HPLC to analyze the monosaccharide compositions in polysaccharides of Dendroblum nobile Lind. from different growth years. Methods The polysaccharide were hydrolyzed into mono- saccharides with TFA. The monosaccharide derivatives obtained with 1 - Phenyl - 3 - methyl - 5 - pyrazolone （PMP） were sepa - rated by HPLC using a gradient elution process. Results The monosaecharide compositions in polysaccharides of Dendrobium nobi- le Lind. from different growth years were significantly differrent. 1 - yeared polysaccharides was composed of mannose, rhamnose, galacturonic acid,glucose, galactose, xylose, and arabinose, with a molar ratio of 68. 33 ： 1. 11 ： 1. 00： 18.96 ： 3.44 ： 5.63 ： 7.26 ;2 - yeared polysaceharides was composed of mannose, galacturonic acid, glucose, galactose, xylose, and arabinose, with a molar ratio of 5.54： 1.07： 26.26： 1.00： 3.15： 5.75. Conclusion This method has accurate result and good reproduc- ibility and can be used to determine the monosaccharides in polysaccharide of Dendrobium nobile Lind. The study provided experi- mental evidence for quality evaluation and development of Dendrobium nobile Lind.

关 键 词：金钗石斛多糖 生长年限 单糖组成 柱前衍生化 高效液相色谱法 

分 类 号：R285.5[医药卫生—中药学]