小分子干扰RNA-紫杉醇固体脂质纳米粒的制备  

Preparation of the solid lipid nanoparticles of siRNA and paclitaxel

作　　者：姚欣宇[1] 石三军[1] 孙逊[1] 

机构地区：[1]四川大学华西药学院 靶向药物与释药系统教育部重点实验室,四川成都610041

出　　处：《华西药学杂志》2014年第4期368-370,共3页West China Journal of Pharmaceutical Sciences

摘　　要：目的制备包裹小分子干扰RNA(siRNA)-紫杉醇(PTX)的固体脂质纳米粒(SLNs)(siRNA-PTX-SLNs),考察其理化性质和包封率。方法采用薄膜超声法制备siRNA-PTX-SLNs;凝胶柱层析法分离SLNs、游离的PTX及游离的siRNA;HPLC法检测PTX的含量;荧光标记法检测siRNA的含量;测定粒径、电位,并在电镜下观察形状。结果 siRNA-PTX-SLNs中紫杉醇的包封率>96%;siRNA的包封率为56.65%±0.5%;SLNs的粒径、电位分别为155±0.23 nm、40±2.34 mV。结论成功制备了能同时有效包裹siRNA和PTX的siRNA-PTX-SLNs。OBJECTIVE To prepare siRNA and paclitaxel loaded solid lipid nanoparticles （SLNs）and investigate its physical and chemical properties and the encapsulation efficiency. METHODS The SLNs were prepared by the film uhrasonication method. SLNs, free FIX and free siRNA were separated by the sephadex filtration. The PTX loaded in SLNs was examined by HPLC. The siRNA encapsulated in SLNs was analyzed by fluorospeetrophotometer. The average diameter, Zeta potential and morphology of SLNs were observed. RESULTS The encapsulation efficiency of PTX was above 96%, and the encapsulation efficiency of siRNA was 56.65 %± 0.5%. The patiele size, PDI and zeta potential of SLNs were 155 ± 0. 23 nm, 0. 214±0. 52 and 40 ±2.34 mV, respectively. CONCLUTION The siRNA -PTX - SLNs were successfully prepared for codelivering siRNA and paclitaxel.

关 键 词：固体脂质纳米粒 凝胶柱层析法 紫杉醇 小分子干扰RNA 高效液相色谱法 

分 类 号：R94[医药卫生—药剂学]