油茶皂素抗人胃癌SGC-7901细胞的活性研究  

Study on antitumor activity of sasanqua saponin in human gastric cancer cell line SGC-7901

作　　者：唐玲[1] 叶立强 王姣[1] 董娟[1] 

机构地区：[1]大连大学生命科学与技术学院,辽宁大连116622

出　　处：《华西药学杂志》2014年第4期371-373,共3页West China Journal of Pharmaceutical Sciences

基　　金：国家自然科学基金资助项目(批准号:30801509)

摘　　要：目的研究油茶皂素7、8对人胃癌SGC-7901细胞的增殖抑制作用及凋亡观察。方法常规培养SGC-7901细胞,采用噻唑蓝(MTT)法检测细胞的活性,而后选用Hoechst33342染色观察细胞的凋亡形态,最后选用AnnexinV/PI双标记法检测细胞的凋亡。结果 5～100μg·mL-1油茶皂素7、20～200μg·mL-1油茶皂素8对人胃癌SGC-7901细胞的抑制作用表现出明显的剂量依赖关系;50μg·mL-1油茶皂素7、100μg·mL-1油茶皂素8处理过的细胞被染色后,部分细胞核内呈现浓染致密的颗粒块状荧光或荧光碎片,表明SGC-7901细胞发生了凋亡;50μg·mL-1油茶皂素7可显著诱导SGC-7901的早期凋亡,早期凋亡率为4.33%,总凋亡率(8.1%)明显大于空白对照组(4.89%),150μg·mL-1油茶皂素8可明显诱导SGC-7901细胞的早期凋亡(2.46%)和晚期凋亡(5.69%)。结论油茶皂素7、8可剂量依赖性抑制SGC-7901细胞的增殖活性,并能诱导SGC-7901细胞凋亡。OBJECTIVE To study the sasanqua saponin 7,8 inhibited the proliferation and apoptosis on human gastric cancer SGC - 7901. METHODS The effect of strian proliferative on SGC -7901 of sasanqua saponin 7,8 by MTT was observe,and Hoechst 33342 staining was chosen and apoptotic debris was found with flow cytometry in SGC -7901. RESULTS Sasanqua saponin 7（5 - 100 μg·mL^- 1 ） and sasanqua saponin 8 （ 20 - 200 μg· mL ^- 1） could significantly inhibit the proliferation in a dose - dependent manner. The part of the nucleus redered stain dense particles block flnrescence or flureseence debris by sasanqua saponin 7 （50 μg·mL^- 1 ）and sasanqua saponin 8 （ 100 μg. mL- 1 ）. 50 μg· mL^- 1 sasanqua saponin 7 could significantly induced early apoptosis of SGC - 7901 （4.33 % ） and otal apoptosis rate （8.1% ） was bigger than control group （4.89%）. 150 μg· mL^-1 sasanqua saponin 8 could induce early apoptosis of SGC -7901 （2.46%） and late apoptosis （5.69%）. CONCLUSION Sasanqua saponin 7,8 can inhibit the proliferation of SGC - 7901 cells in a dose- independdet manner,and induce apoptosis.

关 键 词：油茶皂素7 油茶皂素8 人胃癌SGC-7901细胞 细胞凋亡 

分 类 号：R96[医药卫生—药理学]