HPLC-ELSD法测定黄芪中黄芪皂苷Ⅲ、黄芪皂苷Ⅳ的含量  

Determination of Astragaloside Ⅲ and Astragaloside IV in Astragalus with HPLC-ELSD Method

作　　者：史静超[1] 王永辉[1] 樊思聪[1] 周然[1] 

机构地区：[1]山西中医学院,山西太原030024

出　　处：《世界中西医结合杂志》2014年第7期720-722,共3页World Journal of Integrated Traditional and Western Medicine

基　　金：科技部"十二五"科技支撑计划资助项目(No.2011BAI07B01);山西省科技厅攻关资助项目(No.20110313001-2;No.20120313015-3)

摘　　要：目的：建立HPLC-ELSD法测定黄芪中黄芪皂苷Ⅲ和黄芪皂苷Ⅳ的含量。方法采用Dimonsil C18（150 mm ×4．6 mm，5μm）色谱柱，乙腈-水为流动相梯度洗脱，流速为1 mL·min-1，柱温为30℃；ELSD参数：漂移管温度为100℃，空气流速为2．5 L＆#183;min-1，撞击器关闭。结果黄芪皂苷Ⅲ在进样量范围为0．016～1．150μg时，进样量的自然对数与峰面积积分值的自然对数成良好线性关系（r＝0．9992），平均回收率为98．09％，RSD为1．02％（n＝6）。黄芪皂苷Ⅳ在进样量范围为0．093～0．816μg时，进样量的自然对数与峰面积积分值的自然对数成良好线性关系（r＝0．9993），平均回收率为97．39％，RSD为1．13％（n＝6）。结论本方法简便、准确，可用于黄芪的质量控制。Objective ToestablishHPLC-ELSDmethodforthedeterminationofastragalosideⅢandastragalosideⅣinastragalus.Methods DimonsilC18（150mm×4.6mm,5μm）chromatographiccol-umn was used,acetonitrile-water was as mobile phase for gradient elution,flow rate 1 mL＆#183;min-1 ,column temperature 30 ℃.ELSD parameters：drift tube temperature 100 ℃,air velocity 2.5 L·min-1,impactor closed.Results Insamplesize0.016~1.150μgofastragalosideⅢ,thenaturallogarithmofsamplesize presented good linear relationship with that of peak area value （r =0.999 2 ）,the average recovery was 98.09%,RSD was 1.02%（n=6）.In sample size 0.093 ~0.816 μg of astragaloside Ⅳ,the natural log-arithm of sample size presented good linear relationship with that of peak area value（r=0.999 3）,the aver-agerecoverywas97.39%,RSDwas1.13%（n=6）.Conclusion Thismethodissimpleandaccurateand is applicable for quality control of astragulas.

关 键 词：HPLC-ELSD 黄芪 黄芪皂苷Ⅲ 黄芪皂苷Ⅳ 含量测定 

分 类 号：R927.2[医药卫生—药学]