毛尖紫萼藓(Grimmia pilifera)耐旱相关cDNA文库中4个候选基因的表达分析  

Expression analysis of four candidate genes from drought-tolerant cDNA library of Grimmia pilifera

作　　者：宋晓宏[1] 沙伟[2] 

机构地区：[1]长江师范学院生命科学与技术学院,重庆涪陵408100 [2]齐齐哈尔大学生命科学与工程学院,黑龙江齐齐哈尔161006

出　　处：《江苏农业学报》2014年第2期264-267,共4页Jiangsu Journal of Agricultural Sciences

基　　金：国家自然科学基金项目(31070180;31270254)

摘　　要：采用实时荧光定量PCR方法,分析毛尖紫萼藓中4个干旱胁迫诱导基因在干旱及复水条件下的表达模式。结果表明,GpSuSy基因主要受干旱胁迫诱导表达,GpATP基因和GpPAK基因主要受复水诱导表达,GpCAT基因在干旱及复水条件下均有较高表达量,4个基因在毛尖紫萼藓耐受干旱及复水恢复过程中分别发挥作用。Real-time fluorescence quantitative PCR was used for identification of expression profiles of 4 droughtstress induced genes in Grimmia pilifera under dehydration and rehydration. The expression of GpSuSy was induced bydrought stress, the expressions of GpATP and GpPAK were induced by rehydration, and GpCAT had a high expression levelduring both dehydration and rehydration. These results demonstrated that four genes play roles in drought resistance ofGrimmia pilifera respectively

关 键 词：毛尖紫萼藓 实时荧光定量PCR 表达分析 抗旱性 

分 类 号：S188[农业科学—农业基础科学] Q786[生物学—分子生物学]