小鼠卵母细胞减数分裂期间组蛋白乙酰化及其对卵母细胞成熟质量的影响  

Histone acetylation and its influences on maturation quality during mouse oocyte meiosis

作　　者：王婧[1,2] 李静心[2] 孟春花[2] 朱前明[1,2] 刘勇[3] 曹少先[1,2] 王慧利[2] 

机构地区：[1]南京农业大学动物科技学院,江苏南京210095 [2]江苏省农业科学院畜牧研究所,江苏南京210014 [3]阜阳师范学院生命科学学院,安徽阜阳236041

出　　处：《江苏农业学报》2014年第2期349-354,共6页Jiangsu Journal of Agricultural Sciences

基　　金：国家自然科学基金项目(31101715;31201789);江苏省农业科技自主创新基金项目[CX(12)5012]

摘　　要：为了解卵母细胞减数分裂期间组蛋白乙酰化规律,以组蛋白H4K12的乙酰化为标记,利用免疫荧光技术,研究了小鼠卵母细胞减数分裂期间组蛋白乙酰化的变化规律及其对卵母细胞成熟质量的影响。结果表明:小鼠生发泡期卵母细胞中组蛋白高度乙酰化,在第1及第2次减数分裂中期则发生去乙酰化;II型组蛋白去乙酰基酶(HDACs)负责调控减数分裂过程中的组蛋白乙酰化;减数分裂过程中存在组蛋白乙酰化/去乙酰化的动态变化;特异性抑制II型HDACs不影响卵母细胞成熟、纺锤体形态和染色体排列,但广谱性抑制HDACs后会导致卵母细胞染色体排列发生改变。Using the acetylation of histone H4 at lysine 12 as an indicator, the rule of histone acetylation and itseffects on mouse oocytes maturation quality was studied. Hyperacetylation of histone was observed in germinal vesicle oo-cytes and deacetylation occurred in metaphase I and metaphase II oocytes. Class II HDACs regulated the histone acetylationduring meiosis. Histone acetylation/ deacetylation occurred during meiosis. Specific inhibition of HDACs did not affect oo-cytes maturation, spindle morphology and chromosome alignment, however, inhibition of HDACs with broad-spectrum in-hibitor induced changes in chromosome alignment.

关 键 词：卵母细胞 组蛋白乙酰化 H4K12 纺锤体 染色体 

分 类 号：S814.6[农业科学—畜牧学]