Assay and Evaluation of Flavonoid Content in Chinese Celery  

中国芹菜黄酮含量的测定评价(英文)

作　　者：阎君[1] 于力[1] 许爽[1] 王颖[1] 沈建华 朱为民[1] 

机构地区：[1]上海市农业科学院园艺研究所,上海市设施园艺重点实验室,上海201403 [2]上海星辉蔬菜有限公司,上海201419

出　　处：《Agricultural Science & Technology》2014年第7期1200-1204,共5页农业科学与技术（英文版）

基　　金：Supported by Shanghai Green Vegetable Industrial Technology System;Key Scienceand Technology Research Program of Science and Technology Commission of Shanghai Municipality(12391900900);Innovation Project of Science and Technology Foundation of Pudong New District(PKQ2012-03);Special Fund for Agro-scientific Research in the Public Interest(201403032)~~

摘　　要：Flavonoids were the most important secondary metabolites in celery （Apium graveolens L.）.In this study,the flavonoid （apigenin and luteolin） contents of leaves and petioles in 44 Chinese celery varieties,an American celery variety ‘Ventura＇ and an European celery variety ‘Princer＇ were assayed by reversed-phase high-perfor mance liquid chromatography （RP-HPLC）.The results showed that significant differences in the apigenin and luteolin contents of the leaves and petioles were found among 46 celery varieties,and the variation coefficients of leaf apigenin content,leaf luteolin content,leaf flavonoid content,petiole apigenin content,petiole luteolin content and petiole flavonoid content were 30％,10％,30％,20％,30％ and 20％,respectively.There was no correlation between the leaf flavonoid content and petiole flavonoid content.The apigenin content was about 18 to 50 times of luteolin in celery leaf and about 19 to 40 times of luteolin in celery petiole.The flavonoids in most of the Chinese celery varieties were higher than in ‘Ventura＇ and ‘Princer＇.The flavonoid contents of Chinese celery varieties from the Yangtze Valley and southern China were higher than those from northern China.Our results suggested that Chinese celery varieties with high petiole apigenin contents could be used for vegetable breeding because the petiole was the main edible part,and Chinese celery varieties with high leaf apigenin contents could be used for apigenin production.黄酮类物质是芹菜最重要的次生代谢产物.该研究通过反相高效液相色谱测定了44份中国芹菜种质、美国西芹‘文图拉’和欧洲西芹‘皇后’叶片和叶柄中的芹菜素和毛地黄黄酮的浓度.结果表明：这46份芹菜种质叶片和叶柄中的芹菜素和毛地黄黄酮含量存在显著差异,叶片芹菜素含量、叶片毛地黄黄酮含量、叶片黄酮含量、叶柄芹菜素含量、叶柄毛地黄黄酮含量和叶柄黄酮含量的变异系数分别为30％,10％,30％,20％,30％和20％.叶片和叶柄中的黄酮含量没有相关性.叶片中芹菜素浓度是毛地黄黄酮浓度的18～50倍,叶柄中芹菜素浓度是毛地黄黄酮浓度的19-40倍.大部分中国芹菜种质的黄酮浓度高于西芹‘文图拉’和‘皇后’.分布在中国长江以南地区芹菜种质的黄酮含量高于北部地区芹菜种质.综上所述,叶柄是主要食用部位,因此叶柄具有高芹菜素含量的中国芹菜种质可应用于芹菜的育种,叶片具有高芹菜素含量的中国芹菜种质可应用于生产芹菜素产品.

关 键 词：Chinese celery Flavonoid Reversed-phase High-performance liquid chromatography 

分 类 号：S636.3[农业科学—蔬菜学]