基于ISSR分子标记技术的山核桃幼胚DNA甲基化初步研究  

ISSR analysis of DNA methylation variety on immature embryos of Carya cathayensis

作　　者：李顺福[1,2] 胡恒康[1,2] 张秋露[1,2] 徐艳玲[1,2] 张启香[1,2] 

机构地区：[1]浙江农林大学林业与生物技术学院,浙江临安311300 [2]浙江农林大学亚热带森林培育国家重点实验室培育基地,浙江临安311300

出　　处：《浙江农林大学学报》2014年第4期521-527,共7页Journal of Zhejiang A&F University

基　　金：国家自然科学基金资助项目(31100461);浙江省自然科学基金资助项目(Y3090091)

摘　　要：用简单序列重复区间扩增(inter-simple sequence repeat,ISSR)DNA分子标记技术对山核桃Carya cathayensis不同发育阶段幼胚(第9周至13周)的DNA进行甲基化状态检测。结果表明:17个引物在各个发育时期山核桃幼胚样品中共扩增检测到的128个甲基化位点。甲基化比例随着幼胚的发育逐渐升高,5个时期幼胚甲基化比例依次为23.44%,25.78%,28.90%,40.62%和42.19%。进一步研究表明:5个时期均存在全甲基化位点和半甲基化位点,其中,全甲基化位点所占比例分别为7.81%,8.59%,16.40%,24.22%和25.00%;半甲基化位点所占分别为15.62%,17.19%,12.50%,16.41%,17.19%。因此,山核桃幼胚5个发育时期在基因组DNA甲基化水平上存在着较大差异(P<0.01)。To evaluation the methylation status of different immature embryo stages of Carya cathayensis, DNAmethylation variety was detected on immature embryos of Carya cathayensis, and an inter-simple sequencerepeat （ISSR） analysis was performed to reveal the DNA methylation in five developmental stages of immatureembryos （9 - 13 weeks post pollination）. Results revealed 128 bands obtained from 17 primers. Withdevelopment of the immature embryos, the methylation percentage from 9 to 13 weeks post pollinationincreased gradually from 23.4% to 25.8%, 28.9%, 40.6%, and then 42.2%. Also, total DNA methylation （7.8%,8.6%, 16.4%, 24.2%, and 25.0%） and half DNA methylation （15.6%, 17.2%, 12.5%, 16.4%, and 17.2%）existed. Thus, there was DNA methylation variety with the development of the immature embryos of Caryacathayensis （P〈0.01）.

关 键 词：经济林学 山核桃 幼胚 DNA甲基化 

分 类 号：S664.1[农业科学—果树学]