胸腺肽-α_1通过调节CD_(28)/CD_(152)协同刺激因子促进重症脓毒血症患者外周血T淋巴细胞增殖  

The effect of thymosin-α_1 in regulating the proliferation of peripheral T lymphocytes and expression of co-stimulatory molecules in patients with severe sepsis

作　　者：钟维农[1] 蒋明彦 赵祝香[1] 黄晓梅[1] 赵子文[1] 

机构地区：[1]广州市第一人民医院呼吸内科,广州510180 [2]湖南省湘潭市中心医院,湘潭411100

出　　处：《广州医药》2014年第4期4-6,共3页Guangzhou Medical Journal

基　　金：广东省科技计划(2012B31800327)

摘　　要：目的探讨胸腺肽-α1对重症脓毒血症患者外周血T淋巴细胞增殖的影响及其机制。方法采用细胞膜染料PKH26和荧光标记的特异抗体与流式细胞术结合检测胸腺肽-α1对重症脓毒血症患者外周血T淋巴细胞增殖及CD28/CD152协同刺激因子的表达。结果胸腺肽-α1可促进重症脓毒血症患者外周血T淋巴细胞增殖(P=0.002);可上调CD28+/CD3+的表达及下调CD152+/CD3+的表达。结论胸腺肽-α1通过调节CD28/CD152协同刺激因子促进重症脓毒血症患者外周血T淋巴细胞增殖。Objective To study the effects of Thymosin-α1in regulating the Proliferation of Peripheral T-Lymphocytes and related mechanisms in Patients with severe sepsis.Methods PKH26 and Flow Cytometry were used to detect the level of proliferation in T-lymphocyte and expression of CD28/CD152co-stimulatory molecule in the peripheral blood in Patients with severe sepsis after treated with Thymosin-α1.Results The proliferation of T lymphocytes increased significantly in human peripheral blood after treated with Thymosin-α1（P =0.002）.Meanwhile,Thymosin-α1can improve significantly the level of expression of CD28^＋/CD3^＋on the surface of T-lymphocytes in human peripheral blood（P = 0.041）,but decreased the level of on in the expression of expression of co-stimulatory molecules CD152^＋/CD3^＋（P =0.004）.Conclusion Thymosin-α1 can significantly increase the proliferation of T-lymphocytes in patients with severe sepsis by regulation of CD28/CD152.

关 键 词：重症脓毒血症 胸腺肽-Α1 细胞增殖 协同刺激分子 

分 类 号：R459.7[医药卫生—急诊医学]