SENP-1在脐带血造血干细胞中的表达研究  

A study on the expression of SENP-1 in umbilical cord blood hematopoietic stem cells

作　　者：卞咏梅 刘金宏[2] 谢毅[2] 

机构地区：[1]上海市闵行区妇幼保健院,201102 [2]复旦大学血液病研究中心,200240

出　　处：《中国优生与遗传杂志》2014年第7期11-13,共3页Chinese Journal of Birth Health & Heredity

基　　金：上海市闵行区科委科研基金项目(2012MHZ2069)

摘　　要：目的分析苏素特异蛋白酶1(SENP-1)在脐带血造血干细胞(UCB-HSCs)中的表达情况。方法通过逆转录多聚酶连反应(RT-PCR)法检测SENP-1分子mRNA水平,以?-action为内对照;实验分为对照组A组、B组,实验组C组、D组、E组,每组检测15例标本。结果对照组中A组和B组均未表达SENP-1mRNA,实验组中C组、D组、E组均表达SENP-1类分子mRNA,以SENP-1mRNA积分光密度值/?-actin mRNA积分光密度值比较各组冻存前后及冻存时间长短SENP-1表达差异,C组为0.146±0.011,D组为0.314±0.011,E组为0.368±0.019。结论白血病细胞(HL-60)不表达SENP-1mRNA分子,即使经炎症因子r-干扰素(IFN-r)刺激也不表达,不能有效激活CD8+杀伤T细胞(CTL),是发生免疫逃逸的重要原因;脐带血造血干细胞中均有SENP-1的表达,随着冻存时间越久,SENP-1表达量越下降,表明冻存可以使脐带血干细胞的免疫原性下降。Objective： Study on the expression of SENP-1 in umbilical cord blood hematopoietic stem ceils （UCB-HSCs） . Methods： To examine the level of expression of SENP-lmRNA by RT-PCR. Useing B-action as internal control. The exprimental group was divided into A group and B group, The control group was divided into C group, D group and E group. Each group had 15 cases. Results： There was no expression of SENP-lmRNA in control group and there was obvious expression in exprimental group. Complare the different expressions of SENP-1 in every group by SENP-lmRNA/B-action. The C group was 0.146 _-＋0.011, the D group was 0. 314 _-40.011, the E group was 0. 368 -Z0. 019. Conclusion： The expression of SENP-lmRNA was not detectable in the leukemia cell HL-60, there was no expression even if by the stumilation of IFN-r, so it can＇ t effectively activate CTL cells, this was the important reason of immune escape; the expression of SENP-lmRNA was detectable in every exprimental group, the expression of SENP-lmRNA was decreased when the frozen storage time was increasing, this indicated that frozen storage can make immunogenicity of UCB-HSCs reduced.

关 键 词：脐带血造血干细胞 苏素特异蛋白酶1 逆转录多聚酶连反应 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]