橙皮素对离体大鼠胸主动脉环舒张作用和机制研究  

Effect of Hesperetin on Vasorelaxation of Isolated Thoracic Aortic Rings of Rat

作　　者：李金艳[1] 侯晓敏[1] 李江涛[1] 刘宇[1] 张轩萍[1] 张明升[1] 

机构地区：[1]山西医科大学

出　　处：《中西医结合心脑血管病杂志》2014年第7期870-872,共3页Chinese Journal of Integrative Medicine on Cardio-Cerebrovascular Disease

基　　金：高等学校博士学科点专项科研基金(No.20101417110003);山西省优势学科基金

摘　　要：目的观察橙皮素(HSP)对大鼠离体胸主动脉血管反应性的影响及其可能的机制。方法采用离体血管平滑肌张力实验方法,观察HSP对Sprague Dawley(SD)大鼠离体胸主动脉环的作用,并探讨其作用机制。结果 HSP对氯化钾(KCl)和去氧肾上腺素(PE)预收缩的血管环具有浓度依赖性的舒张作用。去内皮后,HSP不同浓度梯度(10-5 mol/L,3×10-5 mol/L,6×10-5 mol/L,10-4 mol/L)的舒张血管作用减弱。结论 HSP对KCl和PE预收缩大鼠离体胸主动脉环具有浓度依赖性舒张作用。Objective To investigate the effect of hesperetin （HSP）on the isolated thoracic aortic rings of rats and to explore under-lying the mechanism of the HSP.Methods The isolated thoracic aortic rings of male Sprague Dawley （SD）rats were mounted on the organ bath and tension was recorded.The effect of HSP on the isolated thoracic aortic rings with intact endothelium or denuded endo-thelium was precontracted by the potassium chloride （KCl,60 mmol/L）or phenylephrine （PE,10-6 mol/L）,and the effects of HSP on the blood vessels reaction induced by various drugs were recorded with Powerlab biological signal assay system.Results HSP completely vasorelaxed the contractions induced by KCl and PE in a concentration dependent manner in endothelium intact and endo-thelium denuded rat aortic rings.When the contractions was induced by KCl and PE,L NAME （10-4 mol/L）,Indo （10-5 mol/L）and BaCl2 （10-3 mol/L）had no significant inhibition on the vasorelaxant effects of HSP （P〉0.05）.When the contractions was induced by KCl,4 AP （10-3 mol/L）could weaken HSP diastolic function （P〈0.05）.When the contractions was induced by PE,4 AP （10-3 mol/L）could weaken HSP diastolic function （P〈0.05）.Conclusion HSP vasorelaxed the contractions induced by KCl and PE in a concentration dependent manner in endothelium intact and endothelium denuded rat thoracic aortic rings.Its mechanism might be related to the voltage dependent potassium channel （Kv）.

关 键 词：橙皮素 胸主动脉环 血管平滑肌 钾通道 

分 类 号：R285.5[医药卫生—中药学] R256.2[医药卫生—中医学]