Langmuir-Blodgett方法构建聚合物仿细胞外层膜结构涂层  

Preparation of a Copolymer Film with Cell Outer Membrane Mimetic Structure by Langmuir-Blodgett Method

作　　者：杨珊[1,2,3] 宫铭[2,4] 宫永宽[2] 

机构地区：[1]陕西师范大学材料科学与工程学院,西安710062 [2]西北大学化学与材料科学学院,西安710069 [3]渭南师范学院化学与生命科学学院,渭南714099 [4]西安科技大学化学与化工学院,西安710054

出　　处：《高分子学报》2014年第8期1110-1115,共6页Acta Polymerica Sinica

基　　金：国家自然科学基金(基金号20974087;21374087);陕西省自然科学基础研究计划(项目号2013JQ2021);陕西省教育厅科研计划(项目号2013JK0656)资助项目

摘　　要：以盖玻片为基质,采用Langmuir-Blodgett(LB)方法制备含磷酰胆碱基团的两亲性无规共聚物聚(2-甲基丙烯酰氧基乙基磷酰胆碱(MPC)-甲基丙烯酸十八烷基酯(SMA)-γ-甲基丙烯酰氧丙基三甲氧基硅(TSMA)(简称PMST)的单层、双层和三层薄膜.薄膜的表面亲疏水性和表面形貌分别用动态接触角(DCA)和原子力显微镜(AFM)进行测试.结果表明,PMST在盖玻片表面形成了致密的膜层,薄膜的致密程度随层数而增加.在水中前进/后退测试DCA过程中,聚合物薄膜在气相/水相界面存在基团的迁移取向,且膜层内会发生交联.双层膜具有仿细胞外层膜结构,单层和三层膜具有反细胞外层膜结构;双层膜的亲水性比单层和三层膜的要好.The Langmuir-Blodgett （LB） technique was used to prepare thin films with cell outer membrane mimetic structure on the surface of cover glass. A crosslinkable amphiphilic random copolymer PMST,which was synthesized with acrylate monomers 2-methacryloyloxyethyl phosphorylcholine （ MPC ） , stearyl methacrylate （SMA） and 3-trimethoxysilylpropyl methacrylate （TSMA） by free radical polymerization in solution, was applied to prepare the mononlayer,bilayer, and trilayer LB films according to the Y-type deposition mode. The surface hydrophilicity and surface morphology were measured by dynamic contact angles （DCA） and atomic force microscope （AFM） methods. The PMST polymer formed a dense and regular film on the surface of the cover glass, and the density of film increased with the number of layers. The surface hydrophilicity of monolayer and trilayer film was similar, and the bilayer was better than them in hydrophilicity. The difference can be attributed to the different surface structure：the bilayer film has the cell outer membrane mimetic structure and the other two have a reversed structure of the cell outer membrane. Once it contacts with water, the film surface structure will change as a result of the migration and reorientation of surface groups at the gas/water interface.

关 键 词：磷酰胆碱 LB技术 接触角 表面改性 生物材料 

分 类 号：O631.1[理学—高分子化学]