西瓜炭疽病菌产毒条件优化及其生物活性测定  

Toxin-producing Condition Optimization and Biological Activity Determination of Colletotrichum orbiculare

作　　者：唐爽爽[1] 刘志恒[1] 余朝阁[1] 郑川[1] 李健冰 赵廷昌[2] 

机构地区：[1]沈阳农业大学植物保护学院,辽宁沈阳110866 [2]中国农业科学院植物保护研究所,北京100193

出　　处：《中国蔬菜》2014年第5期19-25,共7页China Vegetables

基　　金：国家西甜瓜产业技术体系项目(CARS-26)

摘　　要：针对西瓜炭疽病菌进行了产毒条件优化、粗毒素的生物活性及其理化性质测定的研究。结果表明:不同的培养条件对病原菌粗毒素活性的影响不同,pH值为6的Richard培养液产生的粗毒素的生物活性最强,培养温度25℃、黑暗条件下持续振荡培养20d为最佳产毒条件。粗毒素接种西瓜茎蔓可产生与病菌作用相同的发病症状,对种子胚根生长及种子萌发有抑制作用,对幼苗有致萎作用,说明该粗毒素是西瓜炭疽病菌侵染过程中的主要致病因子。以病菌粗毒素接种10种供试植物,其中甜瓜、西葫芦及草莓发病症状明显,而番茄、茄子及辣椒均未见发病症状。Toxin-producing condition optimization and determination of crude toxin biological activityand its physical,chemical properties were carried out on Colletotrichum orbiculare. The results showed thatdifferent culture conditions had different influences on pathogenic toxin activity. The Richard culture medium withpH 6 ,culture temperature at 25℃,in dark,and shaking continuously for 20 d were the best toxin-producingconditions. The watermelon stem inoculated by crude toxin could produce the same acute symptom as the bacteriadid,which had inhibitory effect on seed radicle growth and seed germination; and also a collapsing role onseedling. That toxin is the main pathogenic factor in the process of watermelon anthrax bacteria infection. Amongthe 10 kinds of selected plants inoculated by crude toxin,the symptoms of muskmelon,summer squash andstrawberry were significant,while no symptoms were seen on tomato,eggplant and pepper.

关 键 词：西瓜炭疽病菌 粗毒素 生物活性 理化性质 

分 类 号：S436.5[农业科学—农业昆虫与害虫防治]