芥菜种质资源遗传多样性和亲缘关系的SRAP分析  

SRAP Analysis of Mustard Germplasm Genetic Diversity and Genetic Relationship

作　　者：李宁[1] 司军[1] 任雪松[1] 宋洪元[1] 李成琼[1] 

机构地区：[1]西南大学园艺园林学院,南方山地园艺学教育部重点实验室,重庆市蔬菜学重点实验室,重庆400715

出　　处：《中国蔬菜》2014年第5期26-33,共8页China Vegetables

基　　金：国家科技支撑计划项目(2012BAD02B01);重庆市农作物良种创新工程资助项目〔CSTC2012GG(80005)〕;星火计划项目(2011GA811001);中央高校基本科研业务费资金资助项目(XDJK2010C068);中央高校基本科研业务费资金资助项目(XDJK2010C069)

摘　　要：利用SRAP 标记对111 份芥菜种质的遗传多样性进行了分析,并记载了其形态特征.从300 对SRAP 引物中筛选出21对多态性明显、条带清晰、反应稳定的引物组合,共扩增出150 条条带,其中106 条为多态性条带,多态性比率为70.67%.SRAP 聚类分析结果表明,111 份芥菜材料的遗传相似系数在0.38～0.89 之间,分为5 大类和若干亚类.对芥菜的叶片性状及根、茎、叶、薹的发育状况等农艺性状进行调查分析,结果表明111 份芥菜材料的遗传相似系数在0.39～1.00 之间,分为4 大类和若干亚类.这两种方法的聚类结果基本一致,表明SRAP 标记可以应用于芥菜种质资源遗传多样性和亲缘关系研究.The paper analyzed the genetic diversity of 111 mustards〔 Brassica juncea（L.）Czern. et Coss.〕materials using SRAP markers,and recorded their morphological characteristics.21 primer combinations with highpolymorphism were screened from 300 SRAP primer combinations.106 polymorphic bands among 150 bands wereamplified by 21 SRAP primer combinations,and the polymorphic rate was 70.67%.The SRAP clustering resultsshowed that the genetic similarity of 111 mustards ranged from 0.38 to 0.89,and 111 mustards were divided into5 groups and several subgroups.The agronomic traits including leaf blade traits,and root,stem,leaf,boltingdevelopment were investigated and analyzed.The results showed that the genetic similarity of 111 mustards rangedfrom 0.39 to 1.00,and they could be divided into 4 groups and several subgroups.These two results were nearly thesame,indicating that SRAP markers could be applied in studying the genetic diversity and relationships of mustardgermplasm resources.

关 键 词：芥菜 农艺性状 SRAP标记 遗传多样性 

分 类 号：S637[农业科学—蔬菜学]