Neuritin蛋白在脑损伤组织中的时相性表达及意义  

Expression of neuritin in the brain tissues after traumatic brain injury

作　　者：蔡云鹏[1] 赵冬[2] 许晖[2] 刘祺[2] 戴晶[2] 朱立仓[2] 何学君 杨传豪[1] 王业忠[2] 

机构地区：[1]石河子大学医学院,832000 [2]石河子大学医学院第一附属医院神经外科,832008

出　　处：《实用医学杂志》2014年第15期2384-2386,共3页The Journal of Practical Medicine

基　　金：石河子大学自然科学重点项目(编号:ZRKX2010ZD03)

摘　　要：目的:检测神经突蛋白(neuritin)在大鼠创伤性脑损伤组织中的时相性表达,探讨其在脑损伤后的意义。方法:42只SD大鼠分为正常组、对照组和实验组,改良Feeney法建立大鼠脑损伤模型,术后分6 h、12 h、24 h、3 d、7 d、14 d共6个亚组。免疫组化、Western-blot检测脑损伤组织中neuritin蛋白表达。结果:免疫组化:实验组neuritin蛋白6 h呈阳性表达,12 h^7 d呈强阳性,明显高于对照组与正常组,14 d呈弱阳性表达。Western-blot:实验组neuritin蛋白6 h稍增高,24 h达高峰,持续到7 d,与对照组、正常组比较差异均有统计学差异(P<0.01),14 d明显降低,较正常组差异仍有统计学意义(P<0.05)。结论:Neuritin蛋白在脑损伤组织中表达明显增高,可能在脑损伤后起着重要作用。Objective To detect time-dependent change of neuritin expression in brain tissues after traumatic brain injury and discuss the effect of neuritin after brain damage occurred. Methods Forty-two rats were divided into normal group, control and experimental group. According to the postoperative time divided into 6 subgroups, including 6 hours group, 12 hours group, 24 hours group, 3 days group, 7 days group and 14 days group. Immunohistochemical and western-blot were used to detected the protein expression levels of neuritin. Results The immunohistochemical staining indicated that the positive expression of neuritin was strong in the cytomembrane and cytoplasms of the neurons, with a higher intensity, 6 hours after the operation. 12 hours after the operation last to the seventh day, the neurons with the strongest positive expression, is significantly higher than control group and normal group, significant decrease on the fourteenth day. The result of western-blot indicated that the level of neuritin protein sharply increased at 6 hours, reached the peak on 24 hours and after lasted to the seventh day, significantly higher than control group and normal group （P 〈 0.01）, significant decrease on the fourteenth day （P 〈 0.05）. Conclusion Up-regnlation of neuritin in cerebral contusion tissues may play an important role after traumatic brain injury.

关 键 词：脑损伤 NEURITIN 

分 类 号：R651.15[医药卫生—外科学]