重组人粒细胞巨噬细胞集落刺激因子联合纳米银对深Ⅱ°烫伤治疗作用的影响  

Effect of recombinant human granulocyte / macrophage colonystimulating factor combined with nano-silver for deep burn degreen Ⅱ about treatment

作　　者：杨景哲[1] 陈凤平[1] 冯欣姝[1] 温海玲[1] 耿琪瑛[1] 

机构地区：[1]承德医学院附属医院烧伤整形科,067000

出　　处：《实用医学杂志》2014年第15期2387-2390,共4页The Journal of Practical Medicine

基　　金：河北省2013年医学科学研究重点课题计划指令性课题(编号:20130024);承德市科学技术研究与发展计划项目(编号:20122164)

摘　　要：目的:研究重组人粒细胞巨噬细胞集落刺激因子(rhGM-CSF)联合纳米银对深Ⅱ°烫伤治疗作用的影响。方法:Wistar大鼠建立深Ⅱ°烫伤模型,分为4组,A组(30只):凡士林纱布覆盖,B组(30只):纳米银覆盖,C组(30只):rhGM-CSF涂抹,D组(30只):rhGM-CSF+纳米银覆盖,伤后第1、4、7、10、14、21天,观察创面愈合,计算愈合率,酶联免疫吸附法测定血清中血管内皮生长因子(VEGF)和表皮生长因子(EGF)水平。结果:第10天各组开始愈合,愈合过程A组炎症反应明显,B、C组适中,D组适度;创面愈合率,第10、14、21天组间差异有统计学意义(P<0.05);VEGF水平,A组第21天达峰值为(25.76±1.46)pg/mL,B、C、D组第14天达峰值[(29.73±1.58),(38.91±2.38),(43.54±1.28)pg/mL],第4、7、10、14、21天组间差异有统计学意义(P<0.05);EGF水平,各组均在第21天达峰值[(0.72±0.14),(0.93±0.13),(1.18±0.16),(1.50±0.15)ng/mL],第7、10、14、21天组间差异有统计学意义(P<0.05)。结论:rhGM-CSF联合纳米银,促进深Ⅱ°烫伤创面愈合,并且优于rhGM-CSF、纳米银单独应用。Objective To observe the effect of recombinant human granulocyte/macrophage colonystimulating factor （rhGM-CSF） combined with nano-silver for deep burn degreen Ⅱ. Methods The burn model were done with Wistar rats. They were randomly divided into four groups, group A（n = 30）： petrolatum treatment, group B（n = 30）： nano-silver treatment, group C（n = 30）： rhGM-CSF treatment, and group D（n = 30）： rhGM-CSF combined with nano-silver treatment. The healing rates of the four groups were observed on postburn day 1, 4, 7, 10, 14, 21. Meanwhile the levels of VEGF and EGF in seams were measured with ELISA. Results All groups started to heal on postbum day 10. Group A had inflammation obviously, and group D moderately. There were significant difference in the healing retes on postbum day 10, 14, 21 between four groups （P 〈 0.05）. The level of VEGF in group A peaked on postbum day 21 （25.76 ± 1.46）pg/mL, but the levels of VEGF in group B, group C and group D peaked on posthurn day 14[（29.73 ± 1.58）pg/mL, （38.91 ± 2.38）pg/mL, （43.54 ± 1.28）pg/mL]. On postbum day 4, 7, 10, 14, 21, there were significant difference（P 〈 0.05）. The level of EGF peaked on postbum day 21 in all groups [（0.72 ± 0.14）ng/mL, （0.93 ±0.13）ng/mL, （1.18 ± 0.16）ng/mL, （1.50 ± 0.15）ng/mL]. There were significant difference on postbum day 7, 10, 14, 21 between four groups （P 〈 0.05）. Conelusiolm rhGM-CSF combined with nano-silver treatment could promote wound healing, and be better than rhGM-CSF and nano-silver singly.

关 键 词：烧伤 重组人粒细胞巨噬细胞集落刺激因子 纳米银 VEGF EGF 

分 类 号：R644[医药卫生—外科学]