安脑片诱导急性移植物抗宿主病小鼠转化生长因子β1表达的实验研究  

Influence of Annao tablet on expression of transforming growth factor beta 1 in acute graft-versus-host disease murine

作　　者：吴顺杰[1] 罗藤[2] 吴远彬[1] 李达[1] 代喜平[1] 胡永珍[1] 康颖[1] 

机构地区：[1]广东省中医院血液科,广州市510120 [2]广州中医药大学生理教研室,510120

出　　处：《实用医学杂志》2014年第15期2390-2393,共4页The Journal of Practical Medicine

摘　　要：目的:观察中药安脑片对急性移植物抗宿主病(aGVHD)小鼠的转化生长因子β1(TGF-β1)表达的影响,以探求其干预aGVHD的机制。方法:SPF级Balb/c雄性小鼠作为供鼠,以SPF级C57BL/6雌性小鼠为受鼠,建立aGVHD模型,随机分为安脑片组和空白组,每组20只。移植后第14天及第30天留取标本,运用ELISA法检测小鼠血清TGF-β1、Western Blot法检测脾脏TGF-β1蛋白的表达及荧光定量PCR法检测脾组织中TGF-β1 mRNA表达。结果:ELISA法检测小鼠血清TGF-β1的结果显示,治疗前两组小鼠血清TGF-β1浓度的差异无统计学意义(P=0.305),治疗后第14及30天,安脑片组小鼠血清TGF-β1的浓度分别为(148.31±7.95)及(183.48±5.91)ng/mL,而空白组为(118.97±2.58)ng/mL,两组相比差异有显著性意义(P=0.000)。Western Blot法检测脾脏TGF-β1蛋白表达的结果显示,治疗后第14天,安脑组小鼠TGF-β1蛋白的IOD/IODβ-actin值为0.33±0.05,治疗后30 d,其值继续上升至0.56±0.04,比值均显著高于空白组(P=0.000)。荧光定量PCR检测脾组织中TGF-β1 mRNA表达的结果显示,治疗后第14及30天,安脑片组小鼠TGF-β1 mRNA表达水平分别为1.24±0.04及2.14±0.33,而空白组小鼠TGF-β1 mRNA的表达较治疗前无明显变化。结论:安脑片可通过提高血清TGF-β1的浓度、提高脾脏TGF-β1蛋白、mRNA表达,有效减轻异体移植小鼠aGVHD的症状和程度。Objective To study the influence of Annao tablet on the expression of transforming growth factor beta 1 （TGF-β1） in acute graft-versus-host disease （aGVHD） murine and to explore the interventional mechanism of TGF-β1 on aGVHD. Methods Hematopoietic stem cells of male Balb/c mice were transplantedto female C57BL/6 mice for the development of aGVHD murine model. Recipient mice were divided into Annao group and blank group randomly and respectively administrated with Annao soup （a kind of Chinese herb） and 0.9% sodium chloride intragastrically. Clinical symptom, survival time and body weight were recorded at 14th and 30th day and some sections of liver, small bowel and skin were taken for histological changes. Serum level of TGF-β1 were measured by enzyme-labeled immunosorbent assay （ELISA）, splenocyte protein of TGF-β1 by Western Blot and TGF-β1 mRNA by fluorescent quantitation polymerase chain reaction （PCR）. Results Serum level of TGF-β1 in both groups had no statistical difference （P = 0.305）, but it rose to （148.31 ± 7.95） ng/mL at 14th day and （183.48 ± 5.91 ） ng/mL at 30th day in Annao group, which had significant difference when compared with that in blank group （P = 0.000）. IOD/IODβ-actin value of TGF-β1 protein in Annao group was 0.33± 0.05 at 14th day and 0.56 ± 0.04 at 30th day, which was higher than that in blank group （P = 0.000） and the expression of TGF-β1 mRNA of splenocyte in Annao group was 1.24 ±0.04 at 14th day and 2.14 ±0.33 at 30th day which was much higher than that in blank group （P = 0.000）. Conclusion Annao tablet helps to relieve symptoms of acute GVHD by raising serum level of TGF-β1 and intensifying expression of protein of TGF-β1 and its mRNA.

关 键 词：移植物抗宿主病 安脑片 转化生长因子Β1 

分 类 号：R285.5[医药卫生—中药学]