多浪羊和卡拉库尔羊卵巢促卵泡素受体的定位研究  

Localization of FSHR on Ovary among Different Sheep

作　　者：王惠娥[1,2] 汪澜[1] 王艳萍[1] 马奎[1] 

机构地区：[1]塔里木大学动物科学学院,新疆阿拉尔843300 [2]新疆生产建设兵团塔里木畜牧科技重点实验室,新疆阿拉尔843300

出　　处：《新疆农业科学》2014年第5期957-962,共6页Xinjiang Agricultural Sciences

基　　金：兵团青年创新资金专项(2011CB001);塔里木大学校长基金(TDZKSS201106)

摘　　要：【目的】探讨多浪羊和卡拉库尔羊卵巢组织中FSHR基因表达变化规律。【方法】采用免疫组织化学SP方法对两种绵羊卵巢组织上FSHR进行定位研究。【结果】FSHR阳性细胞主要表达在颗粒细胞上,在卵母细胞和膜细胞上也有少量表达,两地方品种绵羊卵巢上原始卵泡和闭锁卵泡时期表达差异不显著(P>0.05),而在初级卵泡、次级卵泡时期和成熟卵泡上表达差异显著(P<0.05);对同种绵羊分析时又发现原始卵泡与其他卵泡之间FSHR阳性细胞表达差异显著(P<0.05),成熟卵泡时期阳性细胞表达显著(P<0.05)高于初级和次级卵泡。闭锁卵泡是阳性细胞率与其他各级卵泡差异极显著(P<0.01)。【结论】推测出多浪羊对FSH的敏感性高于卡拉库尔羊,表明FSHR含量越高繁殖率越高。FSHR可能也参与其他因子的调控。[ Objective ] To investigate the ovaries of FSHR gene expression regularity. [ Method ] SP method was used to analyze the immunohistochemical to study the localization of FSHR in the sheep. [ Result] The results showed that： FSHR -immunoreactive cells were mainly loaded by granular cells and were less in oocytes and membrane cells. There was significant difference （ P 〉 0.05 ） between primordial follicles of Duo- lang sheep and Karlakurt sheep. While the FSHR -immunoreactive levels in primary and secondary follicular of Duolang sheep were higher than those of Karakurt sheep （ P 〈 0.05 ） ; On the analysis of the same sheep, FSHR - immunoreactive levels in the primordial follicles were smaller than other follicles （ P 〈 0.05 ）, and FSHR - immunoreactive levels in the mature follicles were significantly higher than the primary and secondary follicular （ P 〈 0.05 ）. [ Conclusion ] It can be concluded that there is a relation between effect of reproduction rate among different local sheep breeds and the distribution of FSH receptor on their ovaries.

关 键 词：多浪羊 卡拉库尔羊 促卵泡素受体 免疫组织化学染色方法 

分 类 号：S813.8[农业科学—畜牧学]