CODEHOP法设计简并引物克隆花羔红点鲑β-肌动蛋白基因及生物信息分析  

Cloning and Characterization Analysis of β-actin Gene Fragment by CODEHOP from Salvelinus malma

作　　者：卢玉婷[1] 张雅斌[2] 吉尚雷[1] 王建超[1] 郑伟[2] 张培军[3] 李月红[1] 

机构地区：[1]吉林农业大学动物科学学院,吉林长春130118 [2]吉林省水产科学研究院,吉林长春130000 [3]吉林省卫生监测检验中心,吉林长春130000

出　　处：《中国畜牧杂志》2014年第15期10-14,共5页Chinese Journal of Animal Science

基　　金：国家自然科学基金(30972191);农业部948项目(2014Z43);吉林省教育厅"十二五"科学技术研究重点项目(201486)

摘　　要：本研究克隆了花羔红点鲑β-肌动蛋白基因,结合生物信息学资源和生物软件的使用,利用CODEHOP法针对特定的氨基酸序列设计简并引物,克隆结果进行同源性分析并构建系统进化树。结果表明:应用此种方法设计出的引物简并度相对较低,Tm值的修改较为灵活,产物特异性强;/3-肌动蛋白具有高度的保守性,比对结果与虹鳟同源性达99.8%,与大马哈鱼同源性达99.3%,与大西洋鲑同源性达99.8%,系统发育关系与传统的分类相一致。Designing a pair of degenerate primers for a specific amino acid by CODEHOP using bioinformatics resources and biology software. β-actin gene were cloned from Salvelinus malma and PCR products＇ homology and phylogenetic tree were analyzed. The results indicated that these degenerate primers degeneracy was relatively lower, Tm value modification more flexible and CODEHOP could be used to obtaining specific RT-PCR product. Salvelinus malma 6- actin characterization analysis suggested this gene was highly conservative, homology comparison with Oncorhynchus mykiss, Oncorhynchus tshawytseha, Salmo salar respectively were 99.8%, 99.3%, 99.8%, respectively. Phylogenetic relationship was consistent with the traditional classification.

关 键 词：简并引物 CODEHOP Β-肌动蛋白 

分 类 号：S961[农业科学—水产养殖]