利用组合策略提高汉逊酵母发酵生产D-阿拉伯糖醇  

An Optimal Combination Strategy to Increase D-arabitol Production by Hansenula polymorpha

作　　者：钱卫东[1] 宁肖肖 赵德志[1] 王兰[1] 李梦媛[1] 陈超[1] 常凯[1] 

机构地区：[1]陕西科技大学生命科学与工程学院,陕西西安710021

出　　处：《安徽农业科学》2014年第23期7726-7728,共3页Journal of Anhui Agricultural Sciences

基　　金：国家自然科学基金项目"利用超远缘遗传转化酵母技术解析龙胆苦苷生物合成关键酶基因"(31100040);陕西省教育厅项目"利用酵母全合成抗癌药物紫杉醇的研究(2013-JK0727)"

摘　　要：[目的]提高多形汉逊酵母发酵生产D-阿拉伯糖醇的产量。[方法]通过正交试验确定了用多形汉逊酵母DL-1发酵生产D-阿拉伯糖醇发酵培养基的最佳组分,进而结合培养温度对发酵过程的影响,获得运用变温策略提高多形汉逊酵母生产D-阿拉伯糖醇的方法。[结果]最优发酵培养基组分为:葡萄糖200 g/L,蛋白胨20 g/L,酵母浸粉12 g/L,Triton X-100 10 g/L,硫酸铵3.0 g/L,七水硫酸镁2.5g/L,磷酸二氢钾2.5 g/L;多形汉逊酵母DL-1的最适生长温度和D-阿拉伯糖醇最适合成温度分别为37℃和34℃;变温调控发酵方法为:将发酵培养基在37℃培养24 h后,升温到48℃继续培养24 h,再降温至34℃继续培养96 h得到发酵液。采用该方法发酵结束后D-阿拉伯糖醇产量为114.92 g/L,比恒温发酵(37℃、48℃、34℃)分别提高了30.25%、208.66%、20.93%。[结论]该方法可以提高多形汉逊酵母发酵生产D-阿拉伯糖醇。[ Objective ] In order to improve the production of D-arabitol by Hansenula polymorpha. [Method] Orthogonal array design method was used to optimize the best components of the fermentation medium, then combined the effects of temperature on D-arabitol yield and cell bi-omass , a method for changing the temperature was established. [Result] The results showed that： the optimal fermentation medium composi-tions were as follows：glucose 200 g/L, peptone 20 g/L, yeast extract 12 g/L, Triton X-100 10 g/L, ammonium sulfate 3.0 g/L, magnesium sulfate heptahydrate 2.5 g/L, potassium dihydrogen phosphate 2.5 g/L;The optimal growth temperature of Hansenula polymorpha DL-1 was 37 ℃, whereas the optimal biosynthesis temperature of D-arabitol was 34 ℃ ; The three-stage temperature-shift method was as follow ： firstly, Hansenula polymorpha DL-1 was cultured in 37 ℃ for 24 h, then cultured in 48 ℃ for another 24 h, and finally further cultured in 34 ℃ for 96 h . The results showed that the yield of D-arabitol was 114.92 g/L, which increased 30.25% , 208.66% and 20.93% than constant tem- perature of 37 ℃ ,48 ℃ and 34℃, respectively. [ Conclusion ] The three-stage temperature control method could be used to increase D-arabi- tol production.

关 键 词：D-阿拉伯糖醇 多形汉逊酵母DL-1 正交试验 变温调控 

分 类 号：S188[农业科学—农业基础科学]