七叶莲挥发油的GC/MS分析和抗炎镇痛作用研究  

Chemical Components and Anti-inflammatory and Analgesic Effects of Schefflera arboricola Hayata by GC-MS

作　　者：章立华[1] 刘力[2] 林小凤 张慧[1] 

机构地区：[1]新疆医科大学药学院,新疆乌鲁木齐830011 [2]中国科学院新疆理化技术研究所,新疆乌鲁木齐830011 [3]漳州175部队医院药剂科,福建漳州363000

出　　处：《安徽农业科学》2014年第23期7732-7735,共4页Journal of Anhui Agricultural Sciences

基　　金：新疆医科大学大学生创新基金(CX2014035;XJC2011117)

摘　　要：[目的]研究福建七叶莲挥发油的化学成分,探讨七叶莲挥发油的抗炎镇痛作用。[方法]采用气相色谱-质谱联用技术对七叶莲挥发油的化学成分进行了分析,结合计算机检索技术对分离的化合物进行鉴定,应用面积归一法计算各成分的相对含量,采用醋酸扭体法、小鼠耳二甲苯致炎肿胀法评价七叶莲挥发油的抗炎镇痛效果。[结果]从七叶莲部分挥发油中共分离出61种成分,并鉴定确认了其中53种主要成分,其中主要化合物和含量分别为4-萜品醇20.40%、(-)-斯巴醇16.01%、氧化石竹烯8.90%等;七叶莲挥发油可明显减少小鼠醋酸所致的扭体次数,可明显缓解小鼠二甲苯所致的耳廓肿胀。[结论]福建七叶莲挥发油中主要含萜品烯、醇类化合物,且其挥发油具有明显抗炎镇痛作用。[Objective] To study chemical constituents of Schefflera arboricola Hayata made in Fujian and explore its anti-inflammatory and analgesic effects. [ Method] Using gas chromatography-mass spectrometry technology, the chemical constituents of Schefflera arboricola Hayata was analyzed. With computer retrieval technology, the separation of compounds was identified. The relative content of each constituents were calculated by area normalization. And anti-inflammatory and analgesic effects of the volatile oils of Schefflera arboricola Hayata were evaluated by methods of acetic acid writhing test and mice ear inflammatory induced by swellingtwomethyl benzene. [ Result] 61 components were isola- ted from volatile oils of Schefflera arboricola Hayata, 58 main components of which were identified, containing 20.40% 4-terpineol, 16.01% （-）-spathulenol, 8.90% caryophyUene oxide and so on. The volatile oils of Schefflera arboricola Hayata can significantly reduce writhing times of the mice induced by acetic acid and significantly relieve the xylene induced ear swelling in mice. [ Conclusion] The volatile oils of Schef- flera arboricola Hayata made in Fujian mainly contain terpinenes and alcohols and have obvious anti-inflammatory and analgesic effects.

关 键 词：七叶莲 挥发油 气相色谱-质谱法 抗炎镇痛作用 

分 类 号：S567[农业科学—中草药栽培]