慢性重型乙型肝炎患者外周血DNT细胞及T细胞亚群的表达及意义  

Expression and significance of double negative T cells and lymphocyte subtype in patients with chronic severe hepatitis B

作　　者：刘坤[1] 杨亚萍[1] 刁青[1] 陈璀[1] 孙谢文[1] 

机构地区：[1]河北省秦皇岛市第三医院肝病科,066001

出　　处：《河北医药》2014年第15期2248-2250,共3页Hebei Medical Journal

摘　　要：目的研究外周血CD+3CD-4CD-8(DNT)细胞及T细胞亚群在慢性重型乙型肝炎患者的表达及意义。方法正常对照者32例、慢性乙型肝炎(CHB)40例、慢性重型乙型肝炎(CSHB)35例患者为研究对象,采用流式细胞仪检测各组外周血DNT细胞及T细胞亚群。结果 CHB组和CSHB组DNT细胞占T淋巴细胞的百分比与对照组比较明显增高(P<0.05);而CSHB组增高程度更为显著,与CHB组比较差异亦有统计学意义(P<0.05)。HBV DNA载量与DNT细胞占T淋巴细胞的百分比呈正相关(r=0.742,P<0.05)。CHB组与对照组比较,仅CD+4亚群明显降低,差异有统计学意义(P<0.05);CSHB组和CHB组比较,CD+4亚群及CD+4/CD+8明显降低,CD+8亚群明显升高,差异有统计学意义(P<0.05)。结论慢性重型乙型肝炎患者DNT细胞显著增高及T细胞亚群比例异常可能是慢性乙型肝炎发生发展的重要因素。Objective To investigate the expression and significance of CD＋3CD-4CD-8（ DNT） cells and lymphocyte subtype in patients with chronic severe hepatitis B. Methods The serum samples from 32 healthy subjects,40 patients with chronic hepatitis（ CHB） and 35 patients with chronic severe hepatitis B（ CSHB） were collected in this study. The DNT cells and lymphocyte subtype in peripheral blood were detected by flow cytometry. Results The percent of DNT cells in CHB and CSHB groups [（ 8. 44 ± 4. 25） % and（ 10. 78 ± 5. 33） %,respectively] were significantly higher than those in control group[（ 6.23 ±3.77） %,P 0.05],moreover,the increase extent in CSHB group was more obvious than that in CHB group（ P〈0. 05）. The HBV DNA load was positively correlated to DNT cells（ r = 0. 742,P〈0. 05）. The CD＋4＋ T lymphocytes in CHB group were significantly decreased,as compared with those in control group（ P〈0. 05）. The percentages of CD＋4T lymphocytes and the ratio of CD＋4/CD＋8in CSHB group were significantly decreased,however,CD＋8T lymphocytes were significantly increased,as compared with those in CHB group（ P〈0. 05）. Conclusion The obvious increase of DNT cells and the abnormality of T lymphocyte subset percentage in patients with chronic severe hepatitis B may be one of important reasons for the pathogenesis and development of chronic hepatitis B.

关 键 词：慢性重型乙型肝炎 DNT细胞 T细胞亚群 

分 类 号：R512.62[医药卫生—内科学]