T细胞亚群偏移与雌激素缺乏骨丢失相关性实验研究  

Experimental study of correlation between T-cell paradigm and bone loss induced by estrogen deficiency

作　　者：高秀莲[1] 邢美云 王丽[3] 郭钰琪[3] 周宪宾[3] 张巧凤[3] 张洪海[3] 姚成芳[3] 姜国胜[3] 李霞[3] 

机构地区：[1]山东省禹城市人民医院检验科,禹城251200 [2]枣庄市薛城区人民医院,枣庄277000 [3]山东省医学科学院基础医学研究所肿瘤免疫与中药免疫重点实验室,济南250062

出　　处：《中国免疫学杂志》2014年第7期870-873,878,共5页Chinese Journal of Immunology

基　　金：国家自然科学基金项目(No.81373670);山东省自主创新成果转化专项(2013ZHZX2A0405);山东省博士基金项目(No.BS2009YY041);山东省科技发展计划项目(No.2012GSF11840);山东省自然科学基金项目(No.ZR2010HQ066);山东省计生委项目(No.[2009]8);山东省计生委项目([2009]8,[2014]25);山东省中管局项目(2013-216,2013-218);济南市科技明星计划项目(济科合字2012)资助

摘　　要：目的:观察雌激素缺乏骨丢过程中T细胞亚群(Th1/Th2/Th17/Treg)变化,探讨其在雌激素缺乏骨丢失中的作用,为临床治疗提供新的靶点与途径。方法:30只BALB/c小鼠随机分为正常组、假手术组及卵巢切除组,各10只。ELISA法检测血清雌二醇(E2)水平,双能X线骨密度仪检测股骨骨密度(BMD),流式细胞仪胞内外双染色法检测脾脏单个核细胞Th1/Th2/Th17/Treg亚群比例。结果:与正常小鼠及假手术小鼠比较,卵巢切除小鼠E2、BMD显著降低(P<0.05),Th1、Th17亚群比例明显升高(P<0.05),Th2、Treg亚群比例明显降低(P<0.05),Th1/Th2比值及Th17/Treg升高(P<0.05);BMD与E2水平、Th2、Treg亚群比例呈显著正相关(P<0.05),与Th1、Th17亚群比例呈显著负相关(P<0.05)。结论:T细胞亚群偏移参与了雌激素缺乏导致的骨丢失,逆转T细胞亚群失衡可能成为防治雌激素缺乏骨丢失的有效靶点与途径。Objective：To demonstrate the relationship between the Th 1/Th2/Th17/Treg paradigm and the bone loss induced by estrogen deficiency and looking for potential target for clinical treatment.Methods：30 BALB/c mice were divided randomly into the normal control group , the sham operation group , and the ovariectomy group.The serum estradiol （ E2 ） was assessed by ELISA.Bone mineral density （BMD） of thigh bone was measured with dual energy X ray absorptiometry.Meanwhile,the T-cell subsets （Th1：CD4^＋TNFα＋, Th2： CD4^＋IL-4^＋, Th17： CD4^＋IL-17 A^＋, Treg： CD4^＋CD25^＋Foxp3^＋） in spleen lymphocytes were analyzed by flow cytometry.Results：Compared with the normal group and the sham operation group , both E2 and BMD in the ovariectomy group decreased significantly （ P〈0.05 ）.The percentage of Th 1 and Th17 subset increased while the percentage of Th 2 and Treg decreased significantly in ovariectomy mice compared with sham operation mice.Correlation analysis showed that BMD was positively related to E 2 level and the percentage of Th 2 and Treg subset;however ,BMD was negatively related to the percentage of Th 1 and Th17 subset （ P〈0.05 ）.Conclusion： Conclusion： T-cell paradigm was involved in the bone loss induced by estrogen deficiency.Modifying T-cell paradigm may become a potential target for reducing bone loss induced by estrogen deficiency .

关 键 词：雌激素缺乏 T细胞亚群 骨代谢 骨丢失 相关性 

分 类 号：R392.6[医药卫生—免疫学]