“温阳补气”针法对实验性自身免疫性重症肌无力大鼠血清IL-12和IL-18表达水平的影响及其作用机制  

Influence of “Warming yang and Nourishing qi” acupuncture treatment in serum IL-12 and IL-18 expression levels in experimental autoimmune myasthenia gravis rats and its mechanism

作　　者：王洪峰[1] 李实[2] 董理[1] 王宇峰[1] 

机构地区：[1]长春中医药大学,长春130117 [2]吉林大学中日联谊医院,长春130033

出　　处：《中国免疫学杂志》2014年第7期909-912,共4页Chinese Journal of Immunology

基　　金：国家自然科学基金(81173344);吉林省科技发展计划项目(20111822)资助

摘　　要：目的：观察“温阳补气”针法对实验性自身免疫性重症肌无力（ EAMG）大鼠血清中IL-12、IL-18表达水平的影响，分析针灸治疗重症肌无力（MG）的作用机制。方法：采用乙酰胆碱受体α1（AchRα1）129～145多肽片段免疫接种雌性Lewis大鼠，建立EAMG模型。随机选取建模成功的EAMG大鼠30只，分为针灸治疗组10只、药物对照组10只及模型对照组10只，并将同期购进未建模的10只大鼠设为空白对照组。针灸治疗组大鼠予以“温阳补气”针法治疗，30 min／次，1次／d，7 d为1疗程，疗程间休息1 d，连续治疗2个疗程；药物对照组予以溴吡斯的明灌胃治疗，18.5 mg／（ kg· d），连续治疗15 d；其余2组不予任何特殊处置，仅作对照观察。治疗后抽取大鼠尾根静脉血，采用ELISA法测定大鼠血清中IL-12和IL-18的表达水平。结果：经治疗后，针灸治疗组、药物对照组与模型对照组比较，两组EAMG大鼠血清的IL-12和IL-18表达水平，均明显降低（ P＜0.05或P＜0.01）；针灸治疗组和药物对照组的组间比较显示，两组EAMG大鼠血清的IL-12和IL-18表达水平无显著性差异（ P＞0.05）。结论：“温阳补气”针法可以降低EAMG大鼠血清IL-12和IL-18的表达水平，从而达到治疗MG的作用，且其治疗效果与溴吡斯的明相近。Objective：To observe the influence of the “Warming yang and Nourishing qi” acupuncture treatment in the serum interleukin-12 （IL-12） and interleukin-18 （IL-18） expression levels,in the rats with experimental autoimmune myasthenis gravis （EAMG） and to analyze the mechanism of acupuncture treatment to myasthenis gravis （MG）.Methods： AchRα1 129-145 peptide fragments were used to immune the female Lewis rats to establish EAMG rat models.30 successful EAMG model rats were randomly selected and divided into acupuncture treatment group ,drug control group and model control group （ n=10 ）;the unmodelled 10 rats purchased at the same period were used as blank control group.The rats in acupuncture treatment group were treated by “Warming yang and Nourishing qi” acupuncture treatment,30 min/time,once a day,7 d for a period,1 d rest between two periods,two periods of continuous treatment.The rats in drug control group were treated by the pyridostigmine lavaged treatment , 18.5 mg/（ kg · d ） , continuous treatment of 15 d.The rats in the other two groups had no any special treatment.After treatment,the blood samples from the vein of tail root of the rats in 4 groups were collected ,and the serum expression levels of IL-12 and IL-18 were detected with ELISA method.Results：After treatment,compared with model control group ,acupuncture treatment and drug control group the serum IL-12 and IL-18 expression level of the EAMG rats were significantly decreased （ P〈0.05 or P〈0.01 ）.Compared between acupuncture treatment group and drug control group showed that ,the serum IL-12 and IL-18 expression level of the EAMG rats were no significant difference （ P〉0.05 ）.Conclusion：“Warming yang and Nourishing qi” acupuncture treatment can reduce the serum IL-12 and IL-18 expression levels of EAMG rats to treat MG ,and the effect of treatment was similar to pyridostigmine.

关 键 词：针灸 实验性自身免疫性重症肌无力( EAMG) IL-12 IL-18 

分 类 号：R245[医药卫生—针灸推拿学]