牡丹皮化学成分的HPLC-QTOFMS分析  

Analysis of chemical constituents of Moutan cortex by HPLC-QTOFMS

作　　者：赵文军[1] 林阳[1] 李鹏飞[2] 刘洋[2] 

机构地区：[1]沈阳军区联勤部药品仪器检验所,辽宁沈阳110026 [2]第二军医大学药学院,上海200433

出　　处：《药学实践杂志》2014年第4期261-265,共5页Journal of Pharmaceutical Practice

摘　　要：目的通过高效液相-单重四级杆-飞行时间质谱(HPLC-QTOFMS)联用技术,对中药牡丹皮的主要化学成分进行有效的鉴定。方法色谱分离采用CAPCELL PAK C18(3.0 mm×100 mm,3μm),流动相组成分别为0.1%甲酸水溶液和乙腈,梯度洗脱,流速为0.5 ml/min;质谱定性采用飞行时间质谱,正负离子模式扫描。结果在优化的液质联用条件下,通过单重四级杆-飞行时间质谱鉴定牡丹皮中48个成分。结论通过HPLC-QTOFMS联用技术,为鉴定牡丹皮中的化学成分建立起了一种快速、高效的分析方法。Objective To analyze the main chemical constituents of Moutan cortex by high performance liquid chromatography-quadrupole-time of flight mass spectrometry（HPLC-QTOFMS）.Methods A CAPCELL PAK C18（3.0 mm ×100 mm,3 μm）column was used to do chromatographic separation.0.1% aqueous formic acid solution and acetonitrile were used as mobile phase composition.Gradient elution was used with a flow rate of 0.5 ml/min; QTOFMS was applied for qualitative analysis under positive and negative ion mode.Results 48 major constituents of Moutan cortex were identified by QTOFMS and structure-relevant fragment ions under the optimized condition.Conclusion A simple and reliable method with the usage of HPLC-QTOFMS was established to identify the main chemical constituents of Moutan cortex.

关 键 词：牡丹皮 超高效液相-单重四级杆-飞行时间质谱 化学成分 

分 类 号：R917[医药卫生—药物分析学]