五华鸡Mx基因A2032G变异位点与禽白血病关联分析  

Association analysis of A2032G mutation of the Mx gene with avian leukosis in Wuhua chickens

作　　者：牛娟娟[1] 张群刚[1] 陈兴勇[1] 盛昌树 耿照玉[1] 

机构地区：[1]安徽农业大学动物科技学院,合肥230036 [2]安徽省芜湖市繁昌县动物疫病预防与控制中心,芜湖241200

出　　处：《安徽农业大学学报》2014年第4期601-604,共4页Journal of Anhui Agricultural University

基　　金：安徽省科技攻关项目(12010302058)资助

摘　　要：Mx基因是具有抗禽流感病毒作用的基因。为探讨Mx基因A2032G变异位点是否与禽白血病有关,本试验采用PCR-RFLP方法,检测了99只56周龄五华鸡Mx基因多态性,并与禽白血病进行关联分析。检测群体Mx基因2032位点存在2个等位基因A、G,频率分别为3.03%和96.97%;两种基因型AG、GG,频率分别为6.06%和93.94%。卡方适合性检验显示,该群体Mx基因2032位点的分布偏离Hardy-Weinberg平衡。分析这2种基因型与禽白血病阳性率的相关性显示,AG型群体禽白血病阳性率为16.67%低于GG型阳性率58.06%。基于上述结论,则阴性群体中AG型个体多于GG型,以40只1日龄禽白血病阴性鸡群为对照,相同方法检测Mx基因2032位点突变率,共检测到3种基因型AA、AG、GG,其频率分别为2.50%、5.00%和92.50%,与上述结论不符。推测五华鸡GG型为禽白血病易感群体,禽白血病阳性检出率可能随日龄而发生变化,且AG基因型的禽白血病易感率低于GG基因型。In order to verify the correlation between the M2032 locus of Mx gene and avian leukosis in Wuhua chickens, PCR-RFLP method was used to analyze the polymorphism of the Mx gene at 2032 locus. The frequencies of allele A and G at that locus in the experimental group were 3.03% and 96.97%, respectively. Two genotypes, AG and GG, were detected in 99 Wuhua chickens, with the frequencies of 6.06% and 93.94%, respectively. Chi-square test showed that the frequencies of the Mx gene were significantly deviated from Hardy-Weinberg equilibrium. The correlation between genotypes and avian leukosis indicated that the positive rate of avian leukosis in group of AG genotype（16.67%） was numerically lower than that of GG genotype（58.06%）. Accordingly, the number of AG genotype would be larger than that of GG genotype. Forty one-day old Wuhua chickens negatively in avian leukosis were used to detect the polymorphism of the Mx gene using the same method described as above; however, frequencies of genotypes AA, AG, GG were 2.50%, 5.00% and 92.50%, respectively, which was inconsistent with the above conclusion. We speculate that the GG genotype of Wuhua chickens might be susceptible to avian leukemia. The positive rate of avian leukosis may vary with age.The susceptibility of avian leucosis of AG genotype would be much lower than that of GG genotype.

关 键 词：五华鸡 MX基因 PCR-RFLP 禽白血病 

分 类 号：S858.31[农业科学—临床兽医学]