银中杨叶片离体再生体系的建立及Gt5GT7基因的遗传转化  

Leaf in vitro regeneration and Gt5GT7 genetic transformation in Populus alba×P. berolinensis

作　　者：徐洪伟[1] 高峰[1] 杨伟新[1] 王晶[1] 周晓馥[1] 

机构地区：[1]吉林师范大学植物资源科学与绿色生产吉林省重点实验室,吉林四平136000

出　　处：《广东农业科学》2014年第13期144-146,I0001,共4页Guangdong Agricultural Sciences

基　　金：国家自然科学基金(31070224)

摘　　要：以银中杨幼嫩枝条为外植体,探究不同预培养方式、激素配比对其叶片离体再生体系的影响.结果表明,枝条在MS营养液中预培养3d为最优方式;不定芽诱导最佳培养基为MS＋TDZ 0.1 mg/L＋NAA 0.02 mg/L,叶片可不经历愈伤组织直接形成丛生芽,最适生根培养基为1/2 MS＋NAA 0.05 mg/L＋IBA 0.01 mg/L＋活性炭0.6 g/L.利用发根农杆菌介导法,建立Gt5GT7基因的银中杨叶片遗传转化体系,叶片GUS染色结果初步证明,Gt5GT7基因成功整合入银中杨叶片,且最适宜侵染的菌液A600值为0.45～0.6.Taking young branches of Populus albaxP, berolinensis as plant material, effects of pre-cultures, cytokinin/ auxin ratio on in vitro regeneration were investigated. Results showed that pre-cultured in MS liquid medium for 3 d was the optimal method. The most suitable bud induction medium was MS＋TDZ 0.1 mg/L＋NAA 0.02 mg/L, in which multiple buds could directly form. The optimal rooting medium was 1/2 MS＋NAA 0.05 mg/L＋IBA 0.01 mg/L＋ active carbon 0.6 g/L. Moreover, Gt5GT7 gene was transformed into regenerative leaves mediated by Agrobacterium rhizogenesis. GUS staining illustrated that transformation system was successfully established. Moreover, the optimal A600 was 0.45-0.6.

关 键 词：银中杨 预培养 激素配比 离体再生 Gt5GT7 遗传转化 

分 类 号：S792.11[农业科学—林木遗传育种] Q785[农业科学—林学]