基于荧光法测定皮革中痕量Cr(Ⅵ)方法的研究  

The Determination of Trace Amount of Cr(Ⅵ) in Leather by Fluorescence Quenching Method

作　　者：史锐[1,2] 张小超[1,2] 陈武勇[1,2] 

机构地区：[1]四川大学制革清洁技术国家工程实验室,四川成都610065 [2]四川大学皮革化学与工程教育部重点实验室,四川成都610065

出　　处：《皮革科学与工程》2014年第4期60-64,共5页Leather Science and Engineering

基　　金：科技部科技型中小企业创新基金(08C26214401265)

摘　　要：通过建立荧光猝灭法来检测皮革中痕量Cr（VI）的方法，为皮革中Cr（VI）的检测提供另一种选择。首先通过分析得到检测的最佳条件：测试温度为20℃；缓冲溶液pH为7．0，用量为2mL；荧光素用量为1．0mL；KI用量为2．0mL；KI与Cr（VI）反应时间为15min；静置时间为15min。同时得到了测定Cr（VI）的回归方程为△F=6．0571C（μg／L）-5．2，线性相关系数r=0．9939，线性范围为4—60μg／L，检出限为8．4μg/L，精密度为2．01％，加标回收率大于91％。利用所建立的荧光猝灭法分析了6个不同Cr（VI）含量的皮革样品，并与紫外分光光度法对比．相对误差小于5％，说明该方法适用于皮革中Cr（VI）含量的测定。In this article, the fluorescence quenching method was introduced to be alternative to determine the trace amounts of Cr （VI） in leather. The final optimal conditions were confirmed as listed： testing temperature as 20 ℃, pH as 7.0 and dosage as 2.0 mL of buffering solution, dosage as 1.0 mL of DCF while 2.0 mL of KI, reaction time as 15 minutes, and standing time as 15 minutes.The resulting linear regression equation of AF= 6.0571 C（μg/L）-5.2 with a relative coefficient of 0.9939 were achieved under these conditions. The determination limit was 8.4 μg/L while the reference stan- dard deviation （RSD） was 2.01%（n=10） with the range of standard Cr（VI） solutions which was 4-60 μg/L.And the recycle rate of the suggested method was above 91% which was reasonable. Subsequently, six leather samples with different content of Cr （VI） were determined using the fluorescence quenching method. The relative error between this method and UV spectrophotometry was less than 5% which showed that the fluorescence quenching method was suitable to the determination of Cr （VI） in leather.

关 键 词：皮革 荧光法 紫外分光光度法 铬 

分 类 号：O657.3[理学—分析化学] TS57[理学—化学]