1株广东鸡源新城疫病毒的分离鉴定和毒力基因分析  

Identification of One Newcastle Disease Viruse Strain and its Virulent Genes

作　　者：黎先伟 钟植文 陈珊 吴晓禅 刘镇明[2] 杨傲冰 

机构地区：[1]广东永顺生物制药股份有限公司,广东广州511356 [2]华南农业大学兽医学院,广东广州510642

出　　处：《广东畜牧兽医科技》2014年第4期34-37,共4页Guangdong Journal of Animal and Veterinary Science

摘　　要：从广东粤东地区某商品蛋鸡场的发病鸡群中分离到1株病毒（YS株）,经血凝和血凝抑制试验确定为新城疫病毒（NDV）.对该分离株的F蛋白氨基酸序列的分析结果表明,其F0裂解位点的氨基酸序列为1nR-R-Q-K-R-F117,且含有101K和121V,符合典型NDV强毒株的分子特点.遗传分析结果显示分离株属于基因Ⅶd亚型.F和HN基因的氨基酸相似性分析表明,分离株与基因Ⅶ型NDV Chicken/China/Shandong/02/2010株的相似性最高,均达到99％,而与常用疫苗株B1、V4、Clone30、Mukteswar和La Sota的相似性则较低,分别在86.8％～ 90.4％和87.2％～88.3％之间,说明分离株与经典的NDV毒株存在一定的差异.In recent years, atypical Newcastle disease was popular in some domestic areas. Samples from some sick commercial layer from eastern Guangdong, were inoculated to 9-11 day-old chicken embryos, and one virus, named YS, was isolated. HA and HI tests showed Newcastle disease virus positive. The result of sequence analysis showed that the amino-acid sequence of the fusion protein cleavage site was 112R-R-Q-K-R-F117, with 101 K and 121 V, a typical sequence ofvelogenic strains. Genetic analysis showed that the isolate belonged to genotype VII. The homology analysis of F and HN gene showed that the isolate strain had highest identities with the genotype VII NDV （Chicken/China/Shandong/02/2010）, both with 99%, but low identities with the common vaccine strains, such as B 1, V4, Clone30, Mukteswar and LaSota, only with 86.8%- 90.4% and 87.2%-88.3%, respectively, which differed from the classical strains of NDV.

关 键 词：分离 基因Ⅶ型 鸡 

分 类 号：S852.659.5[农业科学—基础兽医学]