足月胎膜早破孕妇血浆锌、基质金属蛋白酶9及Ⅳ型胶原的表达及其相关性研究  

The relationship of expression of zinc, matrix metalloproteinases-9 and collagen Ⅳ levels and premature rupture of membranes at term

作　　者：郭延霞[1] 陈燕[1] 韩素新[1] 何艳舫[1] 

机构地区：[1]河北联合大学附属医院妇科,河北省唐山市063000

出　　处：《中国综合临床》2014年第8期817-819,共3页Clinical Medicine of China

基　　金：河北省卫生厅科研经费资助项目（20130065）

摘　　要：目的检测足月胎膜早破孕妇血浆锌、血清及羊水基质金属蛋白酶（MMP）9、血清IV型胶原含量变化。方法选择2012年11月至2013年3月确诊为足月胎膜早破患者30例作为胎膜早破组，另30例在同一时期正常分娩的非胎膜早破患者作为对照组，收集所有病例的母体血液及羊水，血浆锌含量用原子吸收法检测，血清和羊水MMP-9含量用ELISA法检测、血清Ⅳ型胶原含量用上转发光法检测，并对它们之间的相关性进行分析。结果胎膜早破组与对照组比较，孕妇血浆锌、血清Ⅳ型胶原、血清MMP-9、羊水MMP-9比较，差异均有统计学意义[锌：（109．10±16．07）μmol／L与（90．54±10．99）μmol／L，t=-5．22，P=0．000；血清Ⅳ型胶原：（56．86±41．26）μg／L与（88．61±44．87））μg／L，t=2．852，P=0．006；血清MMP-9：（1463．25±483．6）μg／L与（1196．9±357．43）μg/L，t=-2．426，P：0．018；羊水MMP-9：（125．48±67．18）μg／L与（72．64±60．74）μg/L，t=-2．873，P=0．006]。孕妇血浆锌与血清Ⅳ型胶原之间呈负相关（r=-0．261，P=0．044）；孕妇血浆锌、血清MMP-9之间呈正相关（r=0．274，P=0．034）；孕妇血清MMP-9、羊水MMP-9之间呈正相关（r=0．264，P=0．047）；血浆锌与羊水MMP-9、血清Ⅳ型胶原与血清MMP-9、血清Ⅳ型胶原与羊水MMP-9之间均无相关性（r值分别为0．215、-0．172、-0．172，P均〉0．05）。结论足月胎膜早破与母体血浆锌、血清及羊水MMP-9含量升高、血清Ⅳ型胶原含量降低有关。Objective To investigate the changes of zinc in maternal plasma and MMP-9, collagen Ⅳ levels in serum and matrix metalloproteinases-9 （ MMP-9 ） level in amniotic fluid in patients with premature rupture of membranes at term （TPROM）. Methods Thirty cases who were diagnosed as PROM at term during Nov. 2012 to Mar. 2013 were enrolled as case group, and 30 cases delivered during the same time without PROM were enrolled as control group. Maternal blood and amniotic fluid were collected from all the cases. The level of zinc in maternal plasma was measured by atomic absorption method and the levels of MMP-9 in serum and amniotic fluid were detected by enzyme linked immunosorbent assay（ELISA） ,while the level of collagen Ⅳ in serum was measured by up-conversion luminescence method. The relationship among them was analyzed. Results Compared to control group, there were statistically significant difference between TPROM and control groups in terms of the level of zinc, collagen Ⅳ, MMP-9 in serum and MMP-9 in amniotic fluid （zinc： （ 109. 10 ± 16.07 ） μmol/L vs. （90. 54 ± 10. 99 ）μmol/L; t = - 5.22, P 〈 0. 001 ; collagen Ⅳ： （ 56. 86 ± 41.26） μg/L vs. （ 88.61 ± 44. 87 ） μg/L; t = 2. 852, P = 0. 006 ; MbiP-9 in serum： （ 1 463.25± 483.6 ） μg/L vs. （ 1 196. 9 ± 357.43 ） μg/L, t = - 2. 426, P = 0. 018 ; MMP-9 in amniotic fluid ： （ 125.48 ± 67.18 ）μg/L vs. （72. 64 ±60.74）μg/L,t = -2. 873 ,P =0. 006）. Zinc level in maternal plasma and collagen IV in serum had a negative relationship in TPROM （ r = - 0. 261, P = O. 044 ）. Zinc level in maternal plasma and MMP-9 level in serum had a positive relationship in TPROM （ r = O. 274, P = O. 034）. MMP-9 levels in serum and amniotic fluid had a positive relationship in TPROM （ r = 0. 264, P = 0. 047 ） . There were no significant relationship between zinc level in maternal plasma, MMP-9 level in amniotic fluid, collagen IV and MMP-9 levels in serum, collagen IV in serum and MMP-9 in amniotic fluid （

关 键 词：足月胎膜早破 锌 基质金属蛋白酶9 Ⅳ型胶原 

分 类 号：R714.433[医药卫生—妇产科学]