花生叶片DNA快速提取方法的优化  

Optimization of Rapid DNA Extraction Methods from Peanut Leaves

作　　者：王蕾[1] 赵新涛[1] 许梦琦[2] 宿烽[1] 任艳[2] 李双铃[2] 石延茂[2] 袁美[2] 王传堂[2] 

机构地区：[1]青岛科技大学化工学院,山东青岛266042 [2]山东省花生研究所/农业部花生生物学与遗传育种重点实验室,山东青岛266100

出　　处：《山东农业科学》2014年第7期11-14,共4页Shandong Agricultural Sciences

基　　金：山东省博士基金(BS2011NY014);留学人员科技活动择优资助项目(2011);国家科技支撑计划(2011BA35B04);"863"子课题(2013AA102602)

摘　　要：以花生幼叶为试材,对高通量DNA提取法、SDS简易法、TPS法以及TENP法4种不同的DNA提取方法进行比较和优化。结果表明,与其他3种改良方法比较,改良TPS法具有不需液氮磨样、提取速度快、试剂种类少、成本低、DNA溶解速度快、质量较好的优点,提取的DNA可用于花生SSR分析。With the young leaves of peanut as experiment material,four different DNA extraction methods,the high-throughput DNA extraction method,the simple SDS method,TPS method and TENP method,were compared and optimized.The results showed that,compared with the other improved methods,the improved TPS method had the advantages of grinding samples with no liquid nitrogen,faster extraction rate,fewer kinds of reagent,lower cost,rapider DNA solution rate and better DNA quality.The DNA extracted through improved TPS method could be used for SSR analysis of peanut.

关 键 词：花生 基因组DNA 提取方法 幼叶 

分 类 号：S565.201[农业科学—作物学]