HBV-DNA和ALT、TBA水平与HBV感染模式的相关性研究  

Correlation of HBV-DNA load,ALT and TBA levels with hepatitis B virus infection pattern

作　　者：林晖[1] 彭素媚[1] 陈振杰[1] 

机构地区：[1]南京军区福州总医院第一附属医院检验科,福建莆田351100

出　　处：《国际检验医学杂志》2014年第14期1925-1927,共3页International Journal of Laboratory Medicine

摘　　要：目的探讨乙型肝炎病毒(HBV)患者血清HBV-DNA和丙氨酸转氨酶(ALT)、总胆汁酸(TBA)与HBV感染模式的关系以评价HBV感染性。方法对308例患者采用荧光定量PCR法检测血清HBV-DNA,用ELISA方法检测血清乙肝标记物,用连续监测法检测血清ALT,用循环酶法检测血清TBA。结果大三阳(HBsAg、HBeAg、HBcAb阳性者)、小三阳(HBsAg、HBeAb、HBcAb阳性者)和1,5(HBsAg、HBcAb)阳性3种感染模式间HBV-DNA水平和血清ALT活性差异有统计学意义(P<0.05),部分HBeAg阴性患者体内仍存在活跃的病毒复制;在HBeAg阴性患者中HBV-DNA拷贝数与血清ALT水平存在显著正相关关系(P<0.05);3种感染模式间血清TBA水平差异无统计学意义(P>0.05)。结论血清HBV-DNA水平和ALT活性与不同HBV感染模式存在相关性,它们可在一定程度上反映HBV复制及传染性强弱。Objective To explore the correlation of serum HBV-DNA load,transaminase（ALT）and total bile acid （TBA）levels with hepatitis B virus （HBV）infection patterns to assess HBV infectivity.Methods The fluorescent quantitative PCR was used to detect serum HBV-DNA in 308 patients with HBV infection.ELISA was adopted to detect the HBV markers.The continuous mo-nitoring method was adopted to detect serum ALT and the cycling enzymatic method was adopted to detect serum TBA.Results The HBV-DNA content and serum ALT level had staitstical differences among the 3 infection patterns of HBsAb,HBeAg and HB-cAb positive,HBsAg,HBsAb,and HBcAb positive and 1,5 positive （P 〈0.05）,the partial patients with HBeAg negative had the active HBV replication in vivo;HBV-DNA copy number in the patients with HBeAg negative was positively correlated with ALT level （P 〈0.05）.Serum TBA level had no statistical difference among three knds of HBV infection patterns （P 〉0.05）.Conclusion Serum HBV-DNA content and ALT activity have the correlation with the different HBV infection patterns,which could reflect the HBV replication and the infectivity intensity in some extent.

关 键 词：肝炎病毒 乙型 丙氨酸转氨酶 总胆汁酸 

分 类 号：R512.62[医药卫生—内科学]