奈达铂联合β-榄香烯对宫颈癌HeLa细胞增殖和凋亡的影响  

Effect of β-elemene combined with nedaplatin on the proliferation and apoptosis of HeLa cells

作　　者：郑瑾[1,2] 马力天[2,3] 任秦有[2] 刘毅[1] 王彦达[3] 蒋宏祥[3] 荆云涛[3] 杨明会[1] 

机构地区：[1]解放军总医院中医院,北京100085 [2]第四军医大学唐都医院中医科暨中西医结合肿瘤科 [3]第四军医大学

出　　处：《山西医科大学学报》2014年第7期565-569,共5页Journal of Shanxi Medical University

基　　金：国家自然科学基金面上项目(81072973);陕西省中医药临床研究基金资助项目(Lc70)

摘　　要：目的探讨奈达铂(nedaplatin,NDP)联合β-榄香烯(β-elemene)对宫颈癌HeLa细胞增殖和凋亡的影响。方法体外培养宫颈癌HeLa细胞,分别将奈达铂(浓度为7.5,15,30,45,60μg/ml),β-榄香烯(浓度为25,50,100,150,200μg/ml),单独作用于HeLa细胞后24 h、48 h用MTT比色法检测细胞的增殖情况,并选取合适的药物浓度(奈达铂15μg/ml,β-榄香烯150μg/ml),进行联合用药,加药24 h、48 h后用MTT法检测细胞的增殖情况,用流式细胞术检测24 h细胞的凋亡率。结果①MTT法显示不同浓度β-榄香烯作用HeLa细胞24 h、48 h后,其增殖抑制率均明显高于正常对照组(P<0.05)。奈达铂组除24 h时7.5μg/ml和15μg/ml无显著差异外,其余各组增殖抑制率均明显高于相应正常对照组(P<0.05)。②联合用药(奈达铂15μg/ml,β-榄香烯150μg/ml)时,对HeLa细胞的抑制作用显著高于单独用药(P<0.01)。结论奈达铂、β-榄香烯单独或二者联合作用均能抑制HeLa细胞的增殖,且联合应用的作用显著高于单独用药,可协同促进HeLa细胞的凋亡。Objective To investigate the effect of β-elemene combined with nedaplatin on the proliferation and apoptosis of HeLa cells.Methods HeLa cells were treated with different concentrations of β-elemene injection （25,50,100,150,200 μg/ml）and nedaplatin （7.5,15,30,45,60 μg/ml）,respectively.Cell proliferation was measured by MTT after HeLa cells were treated with β-elemene or nedaplatin for 24 h and 48 h.Then,15 μg/ml nedaplatin and 150 μg/ml β-elemene were combined to treat HeLa cells for 24 h and 48 h.Cell proliferation was measured by MTT and cell apoptosis was detected at 24 h by flow cytometry.Results ①Cell proliferation was dramatically inhibited by β-elemene injection （25,50,100,150,200 μg/ml） （P ＜ 0.05）,and the inhibitive rate was higher than in normal control group.Except the concentration of 7.5 μg/ml and 15 μg/ml,nedaplatin injection inhibited cell proliferation at 24 h and 48 h （P ＜ 0.05）.② The inhibition rate of proliferation in HeLa cells after combination therapy was higher than that of monotherapy at 24 h （P ＜ 0.01）.Conclusion Nedaplatin,β-elemene and their combination can inhibit the proliferation of HeLa cells and the effect of the combination is significantly higher than that of monotherapy.What＇ s more,nedaplatin combined with β-elemene has synergistic effect on the apoptosis of HeLa cells

关 键 词：榄香烯 奈达铂 HELA细胞 增殖 凋亡 

分 类 号：R737.33[医药卫生—肿瘤]