大白菜游离小孢子培养技术高效体系的研究  

Studies on High Efficient System for Microspore Culture Technology of Chinese Cabbage

作　　者：李菲[1,2] 张淑江[1] 章时蕃[1] 张慧[1] 孙日飞[1] 张振贤[2] 

机构地区：[1]中国农业科学院蔬菜花卉研究所,北京100081 [2]中国农业大学农学与生物技术学院,北京100193

出　　处：《中国蔬菜》2014年第8期12-16,共5页China Vegetables

基　　金："十二五"农村领域国家科技计划项目(2012AA100202);"十二五"国家科技支撑计划项目(2012BAD02B010);农业部园艺作物生物学与种质创制重点实验室项目

摘　　要：以大白菜吉红82、#534DH群体及#438群体为试材,研究了利用细胞破碎仪机械提取小孢子与人工挤压提取小孢子对大白菜游离小孢子活力及提取量的影响。结果表明:机械提取替代人工挤压可在短时间内同时提取多份样品,并可快速调整小孢子的培养浓度。收集40个适期花蕾于5 mL离心管内,3 000 r·min-1破碎10 s,将提取的小孢子定容于25 mL NLN-13培养基,可确保小孢子浓度在适宜培养范围内,并成功诱导胚胎发生。Taking Chinese cabbage〔Brassica campestris L. ssp. pekinensis（Lour）Olsson〕‘Jihong 82’,some of #534DH and #438 population as testing material,studies the effect of mechanical extraction and artificial extrusion on micro-spore viability and extraction quantity.The result indicates that mechanical extraction can replace the artificial one,get multiple samples during short period of time,and rapidly adjust the concentration of microspores culture.40 suitable buds are collected in 5 mL centrilfuge tubes,3 000 r·min-1 micro-spores gained by 10 s smashing extraction are isolated in 25 mL NLN-13 medium.Thus,micro-spore concentration is insured in suitable culture scope,and successfully induced to embryogenesis.

关 键 词：大白菜 游离小孢子培养 细胞破碎仪 

分 类 号：S634.1[农业科学—蔬菜学]