二轴生物反应器模拟微重力效应研究以及流体中微载体运动分析  

Research of the Simulated Microgravity Effects in Two-axis Rotating Bioreactor and Micro-carrier Movement in the Fluid

作　　者：郭林伟[1] 陈敏[2] 张翔[1] 

机构地区：[1]福建农林大学,福建福州350002 [2]福建省农业科学院,福建福州350003

出　　处：《福建农业学报》2014年第6期592-596,共5页Fujian Journal of Agricultural Sciences

基　　金：福建省自然科学基金项目(2010J01093);国家高技术研究发展计划项目(863计划)(2010AA7035020G)

摘　　要：为了解释旋转生物反应器的微重力效应,研究微重力流体中微载体的运动规律。以有效重力加速度远小于地表重力加速度,微载体在水溶液中的＂近似静止＂这2种方法,验证旋转生物反应器的模拟微重力效应。结合自行研制的旋转生物反应器灌流系统环境,运用等效截面分析法对微载体运动位移和速度进行分析,得出在流体环境下运动规律,并通过计算离心力,得到细胞瓶内氧合膜的理想实验半径。研究表明微载体由中心按一定规律向外径向运动,直至触碰到壁面。为了取得较好试验结果,氧合膜半径为小尺寸参数,范围在0.373 3～0.804 2cm。For studying the simulated microgravity in rotating bioreactor and the movement of micro-carrier in the microgravity fluid, two methods were applied to verify the effect of simulated microgravity in rotating bioreactor.. keeping the effective acceleration of gravity far less than the surface gravity acceleration, or the ＂ approximate static＂ condition for micro-carrier in the aqueous solution. In the self-developed perfusion system in rotating bioreactor, the motion displacement and speed of micro-carrier were analyzed to find out the movement rules of micro-carrier in the aqueous solution, and the ideal radius of membrane oxygenator in cell bottle was decided by centrifugal force calculating. The results showed that micro-carrier moved from center to outside following a certain rule until touching the wall. To get better test results, the radius of membrane oxygenator was set to be a small parameter between 0.3 733--0.8 042 cm.

关 键 词：模拟微重力 灌流系统 微载体运动 

分 类 号：Q693[生物学—生物物理学] O35[理学—流体力学]