脾气虚证2型糖尿病大鼠血液代谢组学研究  

Metabolomics research on spleen qi deficiency syndrome T2DM in rat blood

作　　者：杨宇峰[1] 滕飞[1] 徐娜[1] 石岩[1] 

机构地区：[1]辽宁中医药大学,沈阳110032

出　　处：《中华中医药杂志》2014年第8期2464-2466,I0001,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金："十一五"国家科技支撑计划(No.2007BAI20B103)~~

摘　　要：目的:本研究拟通过复制脾气虚证2型糖尿病大鼠模型,对模型血浆标本进行代谢组学检测,寻找脾气虚证代谢综合征病变的特征性代谢产物,探寻脾气虚证2型糖尿病病变的分子机制,从而揭示其中医证侯本质。方法:健康雄性Wistar大鼠按体质量随机分成两组:空白对照组和模型组,每组6只,模型组复制成2型糖尿病。运用超高压液相色谱-四极杆-时间飞行串联质谱仪技术检测两组血浆样本中的小分子代谢产物,找出具有差异的特征代谢物,进而辨识生物标志物并追溯其来源和代谢途径。结果:脾气虚证2型糖尿病大鼠血液中相关代谢发生显著变化,主要有2-羟基丁酸、赤藓糖、L-丝氨酸、苯丙氨酸、尿苷二磷酸、L-高丝氨酸等水平显著上调,上述物质的集合共同构成了脾气虚证2型糖尿病模型的代谢组学特征,而这些物质正是体内糖类、脂类、蛋白质等代谢紊乱的结果。结论:脾气虚证2型糖尿病的发生与糖类、脂类、蛋白质代谢紊乱有关,这些体内的小分子化合物可能是2型糖尿病中医证候的物质基础。Objective： To research the spleen qi deficiency syndrome T2DM model plasma specimens by metabolomics, detect spleen qi deficiency syndrome T2DM characteristic metabolites, explore the molecular mechanism of spleen deficiency syndrome T2DM lesions and further revealle the essence of spleen deficiency syndrome T2DM. Methods： Spleen deficiency syndrome and T2DM rats were divided randomly into two groups： blank group （n=6） and model group （n=6）. UPLC/Q-TOF-MS technology was used to detecte small molecule metabolites in plasma and find out the characteristic metabolites, so as to identify biomarkers and its origins and metabolic pathway. Results： The metabolites in spleen qi deficiency syndrome T2DM rats＇ blood changed greatly. As the result of metabolic disorders in carbohydrate, fat and protein, 2-hydroxy acid, erythrose, L-Homoserine, phenylalanine, uridine diphosphate, L-Homoserine, which were spleen qi deficiency syndrome T2DM characteristic metabolites, increased significantly. Conclusion： Spleen deficiency syndrome T2DM is associated with carbohydrate, fat, protein metabolic disorder, these small molecule compounds may be the material basis of TCM syndromes of T2DM.

关 键 词：脾气虚证 2型糖尿病 代谢组学 

分 类 号：R259[医药卫生—中西医结合] R-332[医药卫生—中医内科学]