金丹活性部位对心肌缺血再灌注损伤大鼠心肌Bax、Bcl-2蛋白表达的影响  

Effects of active site of hypericum attenuatum choisy and Salvia Miltiorrhiza on protein expression of Bax, Bcl-2 in myocardium of MIRI rats

作　　者：李冀[1] 王艳丽[1] 高彦宇[1] 王珊[1] 何增芬[1] 马育轩[1] 

机构地区：[1]黑龙江中医药大学,哈尔滨150040

出　　处：《中华中医药杂志》2014年第8期2580-2582,共3页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金(No.81173185);黑龙江省自然科学基金重点项目(No.ZD201111);黑龙江省研究生创新科研资金重点项目(No.YJSCX2012-334HLJ);黑龙江中医药大学优秀领军人才支持计划(2012)~~

摘　　要：目的:探讨金丹活性部位对心肌缺血再灌注损伤(MIRI)大鼠心肌Bax、Bcl-2蛋白表达的影响。方法:将金丹活性部位对MIRI大鼠进行干预,应用免疫组化法检测大鼠心肌Bax、Bcl-2表达的变化。结果:与模型组相比,水洗脱组,60%醇洗脱组和预处理组Bax灰度值均有明显升高;Bcl-2灰度值均明显下降(P<0.05,P<0.01)。结论:金丹60%乙醇洗脱组与水洗脱组均能明显上调Bcl-2蛋白表达,下调Bax的蛋白表达,说明其可能通过抑制细胞凋亡而发挥保护MIRI的作用。Objective： To observe the effects of active sites of hypericum attenuatum choisy and Salvia Miltiorrhiza （HS） on the protein expression of Bax, Bcl-2 in myocardium of rats with myocardial ischemia-reperfusion injury. Methods： The rats with myocardial ischemia-reperfusion injury were treated with HS. The immunohistochemistry was used to test the expression of Bax and Bcl-2. Results： Compared with the model group, the grey value of Bax of rats in water elution group, 60% ethanol elution group and the pre-treatment group increased significantly, and the grey value of Bcl-2 in rats in treatment groups all decreased （P〈0.05, P〈0.01）. Conclusion： 60% ethanol elution and water elution of HS could increase the expression of Bcl-2, and decrease the expression of Bax. It showed that HS could protect myocardial ischemia-reperfusion injury by inhibiting the apoptosis of myocardial cells.

关 键 词：心肌缺血再灌注损伤 活性部位 BAX Bcl-2 

分 类 号：R285.5[医药卫生—中药学]