针刀、电针和圆利针对兔膝骨关节炎软骨MMP-1、MMP-3、MMP-13和TIMP-1表达的影响  

Expression of MMP-1, MMP-3, MMP-13, TIMP-1 of cartilage in rabbits with KOA traeted by acupotomy, electro-acupuncture and round-sharp acupuncture

作　　者：黄怡然[1] 金英利[1] 李娜[1] 雷林丹[1] 余飞[1] 李玉波[1] 陶琳[1] 郭长青[1] 

机构地区：[1]北京中医药大学针灸推拿学院,北京100029

出　　处：《中华中医药杂志》2014年第8期2600-2604,共5页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金项目(No.81173333)~~

摘　　要：目的:观察基质金属蛋白酶(MMP)-1、MMP-3、MMP-13和TIMP-1在实验性膝骨关节炎兔软骨中的变化规律。方法:改良后的Videman法左后肢伸直位固定制动法建立模型,通过针刀、电针和圆利针干预,造模后13周取材,采用RT-PCR和Western blot检测软骨MMP相关因子基因和蛋白表达水平。结果:造模后13周,MMP-1 mRNA各组无统计学差异。MMP-1蛋白表达:模型组较正常组显著升高(P<0.05);MMP-3,MMP-13 mRNA和蛋白表达,模型组与电针组较正常组明显升高(P<0.05);针刀组和圆利针组较模型组明显降低(P<0.05);针刀组和圆利针组较电针组显著降低(P<0.05);其中,MMP-3 mRNA圆利针组表达明显高于正常组(P<0.05),电针组明显低于模型组(P<0.05),TIMP-1 mRNA各组高于正常组(P<0.05),电针组显著高于模型组(P<0.05);TIMP-1蛋白表达模型组较正常组明显降低(P<0.05),干预各组较模型组显著升高(P<0.05)。结论:针刀和圆利针对MMP-3、MMP-13 mRNA和蛋白表达有抑制作用,表明其治疗机制可能与调节基质金属蛋白酶家族有关,这与电针治疗关节损伤的机制存在差异。Objective： To observe the variation regularity of the expression of MMP-1, MMP-3, MMP-13 and TIMP-1 of cartilage in rabbits with KOA. Methods： The left knee joint was fixed with piaster bandage in extention position to establish the rabbit model of knee joint injury with the modified videman method. Acupotomy （AP）, electro-acupuncture （EA） and round- sharp acupuncture （RSA） were applied to treat in AP group EA group and RSA group. Expression of MMP-1, MMP-3, MMP-13, TIMP-1 mRNA by RT-PCR and expression of proteins by Western blot tests. Results： Expression of MMP-1 mRNA, there was no significant difference between groups; Expression of MMP-1 protein, in comparison with normal control group, model group increased significantly （P〈0.05）. Expression of MMP-3, MMP-13 mRNA and proteins, in comparison with control group, model group, EA group increased significantly （P〈0.05）; to compare with model group, AP group and RSA group decreased significantly （P〈0.05）, to compare with EA group, AP group and RSA group decreased significantly （P〈0.05）, and RSA group of MMP-3 mRNA is much higer than normal control group （P〈0.05）, MMP-3 mRNA in EA group is much higer than model group （P〈0.05）. In comparison with normal control group after modeling, TIMP-1 mRNA of model group, EA group, AP group and RSA group increased significantly （P〈0.05）, and EA group is much higher than model group （P〈0.05）. Expression of TIMP-1 protein, in comparison with normal control group, model group decreased significantly （P〈0.05）, and AP group, EA group and RSA group are much higer than model group （P〈0.05）. Conclusion： The Acupotomy and round-sharp acupuncture adjusting the MMps family may be one of the therapeutic mechanism,but the therapy mechanism are different from Electro-Acupuncture.

关 键 词：针刀 电针 圆利针 膝骨关节炎 软骨 基质金属蛋白酶 

分 类 号：R245[医药卫生—针灸推拿学]